Pollen/Fruit Syndrome: Clinical Relevance of the Cypress Pollen Allergenic Gibberellin-Regulated Protein
| Autor: | Marie Ange Selva, Youcef Shahali, Jean-Marc Busnel, Denis Charpin, Sanaz Keykhosravi, Inna Mercier, Rihane Arif, Hélène Sénéchal, Nhan Pham-Thi, Tomoyasu Aizawa, Rémy Couderc, Pascal Poncet |
|---|---|
| Přispěvatelé: | CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Université Paris-Sud - Paris 11 (UP11), Razi Vaccine and Serum Research Institute [Iran] (RVSRI), Hokkaido University [Sapporo, Japan], Beckman Coulter Life Sciences, Research Department, Centre Médical de l'Institut Pasteur (CMIP), Institut Pasteur [Paris] (IP), Hôpital de la Timone [CHU - APHM] (TIMONE), Centre de Ressources et de Recherche Technologique - Center for Technological Resources and Research (C2RT) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Pulmonary and Respiratory Medicine
[SDV]Life Sciences [q-bio] Immunology Basophil Brief Communication Immunoglobulin E medicine.disease_cause Microbiology peach 03 medical and health sciences pomegranate basophil 0302 clinical medicine Allergen Pollen otorhinolaryngologic diseases medicine Immunology and Allergy [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] [SDV.IMM.ALL]Life Sciences [q-bio]/Immunology/Allergology 030223 otorhinolaryngology Cypress Sensitization [SDV.EE.SANT]Life Sciences [q-bio]/Ecology environment/Health biology CD63 business.industry food and beverages Basophil activation medicine.anatomical_structure 030228 respiratory system pollen biology.protein business allergen |
| Zdroj: | Allergy, Asthma & Immunology Research Allergy, Asthma & Immunology Research, 2019, 11 (1), pp.143-151. ⟨10.4168/aair.2019.11.1.143⟩ |
| ISSN: | 2092-7355 2092-7363 |
| DOI: | 10.4168/aair.2019.11.1.143⟩ |
| Popis: | International audience; A pollen/food-associated syndrome (PFAS) has been described between peach and cypress pollen. Cross-reactive allergens were characterized which belong to the Gibberellinregulated protein (GRP) family, BP14 in cypress pollen and Pru p 7 in peach. GRP are small cationic protein with anti-microbial properties. A patient suffering from a peach/cypress syndrome was explored clinically and biologically using 2 types of immunoglobulin E (IgE) multiarray microchip, immunoblots and a basophil activation test to assess the clinical relevance of various extracts and purified allergens from fruits or cypress pollen. In addition to PR10 sensitization, the patient showed specific IgE to Pru p 7, BP14 and allergen from pomegranate. These last 3 allergens and allergenic sources are able to induce ex vivo basophil activation characterized by the monitoring of the expression of CD63 and CD203c, both cell surface markers correlated with a basophil mediator release. Up to 100% of cells expressed CD203c at 50 ng/mL of BP14 protein. In contrast, snakin-1, a GRP from potato sharing 82% sequence identity with Pru p 7 did not activate patient's basophils. These results strongly suggest that, like Pru p 7, BP14 is a clinically relevant allergenic GRP from pollen. Allergen members of this newly described protein family are good candidates for PFAS where no cross-reactive allergens have been characterized. |
| Databáze: | OpenAIRE |
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