Fluorescence microscopy beyond the diffraction limit
| Autor: | Mike Heilemann |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Fluorescence microscopy
Structured-illumination microscopy Diffraction Microscopy Photoswitching microscopy Photochemistry Super-resolution microscopy Chemistry Resolution (electron density) RESOLFT Analytical chemistry Scanning confocal electron microscopy Bioengineering Nanotechnology General Medicine Applied Microbiology and Biotechnology Microscopy Fluorescence Subdiffraction resolution Stimulated-emission depletion Super-resolution Fluorescence microscope Photoactivated localization microscopy Biotechnology |
| Zdroj: | Journal of Biotechnology. 149:243-251 |
| ISSN: | 0168-1656 |
| DOI: | 10.1016/j.jbiotec.2010.03.012 |
| Popis: | In the recent past, a variety of fluorescence microscopy methods emerged that proved to bypass a fundamental limit in light microscopy, the diffraction barrier. Among diverse methods that provide subdiffraction spatial resolution, far-field microscopic techniques are in particular important as they can be operated in complex biological samples such as cells or tissue. Valuable new insights into biomolecular structure, organization and even dynamic processes in living cells have been gained with these novel microscopic techniques. In the present review, the most important concepts of far-field microscopy with subdiffraction resolution are introduced. The underlying physical concepts are discussed, and practical considerations for the application of these methods are made. (C) 2010 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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