Natural killer cells protect against mucosal and systemic infection with the enteric pathogen Citrobacter rodentium
| Autor: | Silvia Melgar, Fergus Shanahan, Aoife Quinlan, Kenneth Nally, Lindsay J. Hall, Carola T. Murphy, Grainne Hurley |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Colon
medicine.medical_treatment Immunology Context (language use) Inflammation Biology Microbiology Mice Immune system Citrobacter rodentium medicine Cytotoxic T cell Animals Immunologic Factors Pathogen Cecum Host Response and Inflammation Mucous Membrane Enterobacteriaceae Infections Killer Cells Natural Mice Inbred C57BL Infectious Diseases Cytokine biology.protein Cytokines Parasitology Female Antibody medicine.symptom |
| Zdroj: | Infection and immunity. 81(2) |
| ISSN: | 1098-5522 |
| Popis: | Natural killer (NK) cells are traditionally considered in the context of tumor surveillance and viral defense, but their role in bacterial infections, particularly those caused by enteric pathogens, is less clear. C57BL/6 mice were orally gavaged with Citrobacter rodentium , a murine pathogen related to human diarrheagenic Escherichia coli . We used polyclonal anti-asialo GM1 antibody to actively deplete NK cells in vivo . Bioluminescent imaging and direct counts were used to follow infection. Flow cytometry and immunofluorescence microscopy were used to analyze immune responses. During C. rodentium infection, NK cells were recruited to mucosal tissues, where they expressed a diversity of immune-modulatory factors. Depletion of NK cells led to higher bacterial loads but less severe colonic inflammation, associated with reduced immune cell recruitment and lower cytokine levels. NK cell-depleted mice also developed disseminated systemic infection, unlike control infected mice. NK cells were also cytotoxic to C. rodentium in vitro . |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|