Antimicrobial susceptibilities and random amplified polymorphic DNA-PCR fingerprint characterization of Lactococcus lactis ssp. lactis and Lactococcus garvieae isolated from bovine intramammary infections
| Autor: | F.L. Welcome, N. Rota, Daryl V. Nydam, Brenda G. Werner, James Bennett, A. Barberio, Patrick L. McDonough, Rebecca J. Franklin-Guild, Gloria Gioia, Carme Plumed-Ferrer, Paolo Moroni |
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| Rok vydání: | 2015 |
| Předmět: |
medicine.drug_class
Antibiotics Microbial Sensitivity Tests Penicillins Microbiology Minimum inhibitory concentration Mammary Glands Animal Ampicillin Drug Resistance Multiple Bacterial Cefazolin Lactococcus Genetics medicine Animals Mastitis Bovine biology Lactococcus lactis food and beverages Amoxicillin biology.organism_classification Virology RAPD Anti-Bacterial Agents Random Amplified Polymorphic DNA Technique Penicillin Lactococcus garvieae Animal Science and Zoology Cattle Food Science medicine.drug |
| Zdroj: | Journal of dairy science. 98(9) |
| ISSN: | 1525-3198 |
| Popis: | In total, 181 streptococci-like bacteria isolated from intramammary infections (IMI) were submitted by a veterinary clinic to Quality Milk Production Services (QMPS, Cornell University, Ithaca, NY). The isolates were characterized by sequence analysis, and 46 Lactococcus lactis ssp. lactis and 47 Lactococcus garvieae were tested for susceptibility to 17 antibiotics. No resistant strains were found for β-lactam antibiotics widely used in clinical practice (penicillin, ampicillin, and amoxicillin), and all minimum inhibitory concentrations (MIC) were far from the resistance breakpoints. Eight strains had MIC intermediate to cefazolin. The random amplification of polymorphic DNA (RAPD)-PCR fingerprint patterns showed a slightly higher heterogeneity for Lc. lactis ssp. lactis isolates than for Lc. garvieae isolates. |
| Databáze: | OpenAIRE |
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