Discrimination of human CD4 T cell clones based on their reactivity with antigen-presenting T cells
| Autor: | Tony Wyss-Coray, Karin Frutig, Werner J. Pichler, Christian Brander |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte HLA-DP Antigens Time Factors T cell T-Lymphocytes Immunology Antigen presentation CD1 Antigen-Presenting Cells Biology CD5 Antigens Lymphocyte Activation Interleukin 21 CD28 Antigens Antigens CD medicine Immunology and Allergy Cytotoxic T cell Humans Antigen-presenting cell HLA-DP beta-Chains Natural killer T cell Virology Molecular biology Clone Cells medicine.anatomical_structure Phenotype CD4 Antigens Cytokines Calcium CD8 |
| Zdroj: | European journal of immunology. 22(9) |
| ISSN: | 0014-2980 |
| Popis: | In this report, we describe the discrimination of human T cell clones based on their reactivity with activated T cells as antigen-presenting cells (APC). CD4+ T cell clones specific for peptide P30 of tetanus toxin (amino acids 947-967) and restricted to the DP4 molecule were established and tested for proliferation to peptide presented either by peripheral blood mononuclear cells (PBMC), Epstein-Barr virus (EBV)-transformed B cells or major histocompatibility complex (MHC) class II-expressing T cells. We found two sets of T cell clones: one set proliferated to peptide presentation by PBMC, EBV-transformed B cell lines (EBV-B cells) and MHC class II+ T cells (termed T-responder clones), while the other set of clones was only stimulated to proliferate, if the peptide was presented by PBMC or EBV-B cells, but not by T cells (T-nonresponder clones). Nevertheless, these T-nonresponder clones recognized P30 also on T cells, as revealed by Ca2+ influx. The discrimination of the clones was not due to different avidities of the T cell receptors (TcR) of individual clones for the MHC-peptide complex as T-responder and T-nonresponder clones had similar dose-response curves to P30 presented by fixed EBV-B cell lines. Addition of cytokines [interleukin (IL)-1, IL-2, IL-4 and interferon gamma] did not change the proliferative response of the clones, which was consistent throughout an observation period of greater than 4 months. T-nonresponder clones, exposed to P30 on MHC class II-expressing T cells, became not anergic, as they could be restimulated by P30 presented on EBV-B cells. The measurement of a panel of T cell activation markers and adhesion molecules on T-responder and T-nonresponder clones revealed a higher expression of the CD28 molecule on the T-nonresponder clones. The data suggest that freshly cloned T cells can be differentiated by peptide presentation on classical (PBMC, EBV-B cells) or non-classical APC (class II+ T cells), and that this discrimination is further underlined by different levels of adhesion molecules. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text