A simple non invasive computerized method for the assessment of bone repair within osteoconductive porous bioceramic grafts
| Autor: | Barbara Canesi, Silvia Scaglione, Carmela Valastro, Maddalena Mastrogiacomo, Francesco Beltrame, Rodolfo Quarto, Antonio Crovace, Federica Viti, Ranieri Cancedda |
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| Rok vydání: | 2005 |
| Předmět: |
Ceramics
Bone Regeneration Bone density Computer science Radiography Bioengineering Bone healing Bone tissue Applied Microbiology and Biotechnology osteoconductive grafts Sensitivity and Specificity Osseointegration Bone remodeling Absorptiometry Photon Bone Density medicine Animals Bone regeneration bone formation Fracture Healing Sheep business.industry X-ray imaging Reproducibility of Results calibration X-ray imaging engineered bone tissue calibration bone formation osteoconductive grafts Tibial Fractures medicine.anatomical_structure Treatment Outcome Bone Substitutes Radiographic Image Interpretation Computer-Assisted Female Implant business engineered bone tissue Porosity Algorithms Biotechnology Biomedical engineering |
| Zdroj: | Biotechnology and bioengineering. 92(2) |
| ISSN: | 0006-3592 |
| Popis: | Single energy X-ray imaging, due to its low cost and flexibility, is one of the most used and common technique to assess bone state and bone remodeling over time. Standardized X-ray images are needed to compare sets of radiographs for semi-quantitative analyses of tissue remodeling. However, useful mathematical modeling for the analysis of high level radiographic images are not easily available. In order to propose a useful evaluation tool to a wide clinical scenario, we present an innovative calibration algorithm for a semi-quantitative analysis of non-standardized digitized X-ray images. For calibration on a unique standardization scale, three time invariant regions (ROI) of radiographs were selected and analyzed. The accuracy of the normalization method for X-ray films was successfully validated by using an aluminum step wedge for routine X-ray exposures as tool to standardize serial radiographs (Pearson correlation test: R(2) = 0.96). This method was applied to investigate the progression of the new bone deposition within ceramic scaffolds used as osteoconductive substitute in large bone defects taking advantage of a large animal model. This innovative image-processing algorithm allowed the identification and semi-quantification of the bone matrix deposited within the implant. The osteo-integration at the bone-implant interface was also investigated. A progressively increasing bone tissue deposition within the porous bioceramic implant and a progressive osteo-integration was observed during the 12 months of the trial. |
| Databáze: | OpenAIRE |
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