Development of multidrug resistance type I Cmdr1 expression in chicken embryonic gonads
| Autor: | Shinya Aramaki, S. Okuno, Nobuhiko Yamauchi, K. Yamada, Tomoki Soh, Masa-aki Hattori, N. Shibuya |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Male
endocrine system Embryo Nonmammalian Physiology Embryonic Development Ovary In situ hybridization Biology Biochemistry Marker gene Testis medicine Animals ATP Binding Cassette Transporter Subfamily B Member 1 RNA Messenger Gonads Molecular Biology Sexual differentiation Gene Expression Profiling Embryogenesis Embryo Molecular biology Embryonic stem cell medicine.anatomical_structure Gene Expression Regulation Theca Theca Cells Female Chickens |
| Zdroj: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 147:928-933 |
| ISSN: | 1095-6433 |
| DOI: | 10.1016/j.cbpa.2007.02.024 |
| Popis: | A primary role of P-glycoprotein (P-gp), encoded by the multidrug resistance type I gene, is to protect against naturally occurring xenotoxics. Recently, the preferential expression of chicken multidrug resistance type I (Cmdr1) was identified in the embryonic gonads during the early periods of development. Here we investigated the expression of Cmdr1 and P-gp in the gonads during embryogenesis, and compared to that in the ovarian follicles of domestic hens (Gallus gallus). As revealed by immunohistochemistry, P-gp was highly expressed in theca cells of mature follicles, whereas the expression was low in immature follicles. Immunohistochemical analysis showed that expression of Cmdr1-type P-gp was very low in embryonic gonads. Cmdr1 mRNA was undetectable in the gonads of 5-day embryos (E5) by RT-PCR, whereas Cmdr1 mRNA was significantly detectable in the developing gonads at E9 and E21. In the testicular tissues, germ cells were distributed along developing seminiferous cords as identified by a specific marker gene, whereas Cmdr1-type P-gp positive cells were observed evenly on testicular tissues. Collectively, it is concluded that Cmdr1 expression is initiated in the chicken ovary and testis after sexual differentiation, but expression of Cmdr1-type P-gp is very low through embryogenesis. |
| Databáze: | OpenAIRE |
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