Prostaglandin D2 expression is prognostic in high‑grade serous ovarian cancer
| Autor: | Alexandre Andre Balieiro Anastacio da Costa, Nayra Soares do Amaral, Louise De Brot, Katia C. Carvalho, Glauco Baiocchi, Felipe Ilelis de Barros Silva, Rafael Malagoli Rocha, Fabio Albuquerque Marchi, Mariana Rezende Alves, Fernando Augusto Soares |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Oncology Adult Cancer Research medicine.medical_specialty Ovariectomy Kaplan-Meier Estimate Disease-Free Survival 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Biomarkers Tumor Humans Aged Ovarian Neoplasms biology Proportional hazards model Microarray analysis techniques business.industry Prostaglandin D2 Hazard ratio Ovary Prostaglandin D2 synthase Cancer General Medicine Cytoreduction Surgical Procedures Middle Aged Debulking medicine.disease Prognosis Lipocalins Cystadenocarcinoma Serous Intramolecular Oxidoreductases Serous fluid 030104 developmental biology 030220 oncology & carcinogenesis biology.protein Immunohistochemistry Female Neoplasm Grading business Follow-Up Studies |
| Zdroj: | Oncology reports. 41(4) |
| ISSN: | 1791-2431 |
| Popis: | To identify biomarkers that could predict response or lack of response to conventional chemotherapy at the time of diagnosis of high‑grade serous ovarian carcinoma (HGSOC), the present study compared large‑scale gene expression from patients with short or long disease‑free survival times, according to the last cycle of chemotherapy, and validated these findings using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and conventional immunohistochemical (IHC) analysis. Samples were selected for microarray evaluation, at the time of diagnosis, using the following criteria: Identical debulking primary surgery, International Federation of Gynaecology and Obstetrics staging, histological subtype and grade. These were divided into 2 groups, regarding the outcome after 2 years of follow-up. Prostaglandin D2 synthase 21 kDa (brain) (PTGDS) was found to be expressed at a significantly higher level in the tumours of patients with a short disease‑free survival time, and this was validated by RT‑qPCR in all samples. Furthermore, the study evaluated PGD2, the protein product of the PTGDS gene, in a large cohort of 114 HGSOC patients using the Ventana Benchmark automated platform, and IHC positivity was correlated with clinicopathological data and outcome. The global gene expression analysis identified 1,149 genes that were differentially expressed in microarray data, according to the patient outcome. Further analysis RT‑qPCR validated PTGDS gene expression in the same samples (r=0.945; P |
| Databáze: | OpenAIRE |
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