Increase in sensitivity to soluble CD4 by primary HIV type 1 isolates after passage through C8166 cells: association with sequence differences in the first constant (C1) region of glycoprotein 120
| Autor: | M S Kennedy, Paul J. Maddon, J S McDougal, G P Allaway, S L Orloff, C I Bandea |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Male
T cell Immunology Molecular Sequence Data Biology HIV Envelope Protein gp120 Giant Cells Polymerase Chain Reaction Neutralization Virus law.invention Cell Line Antigen Proviruses law Antigens CD Virology HIV Seropositivity medicine Humans Amino Acid Sequence Lymphocytes Peptide sequence Polymerase chain reaction Cells Cultured Phenotype Kinetics Infectious Diseases medicine.anatomical_structure Cell culture CD4 Antigens DNA Viral HIV-1 |
| Zdroj: | AIDS research and human retroviruses. 11(3) |
| ISSN: | 0889-2229 |
| Popis: | Primary isolates of human immunodeficiency virus type 1 (HIV-1) were obtained by coculture of peripheral blood lymphocytes (PBLs) from HIV-1-infected people with PBLs from uninfected donors. These viral stocks tend to be resistant to neutralization/inactivation by soluble CD4 (sCD4). When these stocks were passed through the T cell line C8166, virus stocks emerged that were sensitive to sCD4. Pre- and post-C8166 stocks maintained their sCD4-resistant and -sensitive phenotypes, respectively, with further passage in PBLs. Pre- and post-C8166 stocks were biologically cloned by two cycles of limiting dilution. The majority (14 of 17) of pre-C8166 clones were sCD4 resistant, and, conversely, the majority of post-C8166 clones (11 of 12) were sensitive to sCD4. Nucleotide and predicted amino acid sequence analysis in the env (gp120) region revealed a limited number of differences between the clones. The only differences that sorted with biological phenotype were in the first constant (C1) region of gp120. Adaptation to growth in C8166 cells and conversion from the sCD4-resistant to the sCD4-sensitive phenotype represent the emergence to prominence of viral species in the pre-C8166 stock that have a replication advantage in C8166 coincident with increased sensitivity to sCD4. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|