Comprehensive chromosome screening of polar bodies and blastocysts from couples experiencing repeated implantation failure
| Autor: | S Alfarawati, Dagan Wells, R. Prates, Mandy G. Katz-Jaffe, William B. Schoolcraft, John Stevens, C. Gutiérrez-Mateo, S. Tormasi, N. Goodall, Elpida Fragouli, Santiago Munné, Pere Colls |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male medicine.medical_specialty Abortion Habitual Pregnancy Rate medicine.medical_treatment Aneuploidy Biology Preimplantation genetic diagnosis Andrology Embryo Culture Techniques Polar body Pregnancy medicine Chromosomes Human Humans Blastocyst Embryo Implantation Cells Cultured Preimplantation Diagnosis Gynecology Comparative Genomic Hybridization Family Characteristics In vitro fertilisation Blastocyst Transfer Obstetrics and Gynecology medicine.disease Pregnancy rate medicine.anatomical_structure Reproductive Medicine embryonic structures Cytogenetic Analysis Embryo Loss Female |
| Zdroj: | Fertility and sterility. 94(3) |
| ISSN: | 1556-5653 |
| Popis: | Objective To identify and transfer cytogenetically normal embryos after screening all chromosomes of first and second polar bodies (PBs) or trophectoderm samples with the use of comparative genomic hybridization. Design Clinical research study. Setting In vitro fertilization clinic referring samples to a specialist preimplantation genetic diagnosis laboratory. Patient(s) Thirty-two couples with repeated implantation failure. Intervention(s) Zygotes from patients with repeated implantation failure and poor response to ovarian stimulation underwent PB biopsy. Patients with repeated implantation failure who were candidates for blastocyst transfer received trophectoderm biopsy. Zygotes or blastocysts were vitrified while chromosome analysis took place. Euploid embryos were transferred during a subsequent cycle. Main Outcome Measure(s) Cytogenetic status and implantation and pregnancy rates. Result(s) The oocyte and blastocyst aneuploidy rates were 65.5% and 45.2%, respectively. Abnormalities affecting all chromosomes were detected. Implantation and pregnancy rates for the patients with PB biopsy were 11.5% and 21.4%, respectively, whereas for patients receiving blastocyst analysis they were 58.3% and 69.2%. Conclusion(s) Initial results for patients of advanced maternal age (39.8 years) with repeated implantation failure and poor ovarian response were encouraging. However, further study is required to confirm whether or not screening is beneficial. Blastocyst analysis was associated with high pregnancy rates, suggesting that comprehensive chromosome screening may assist patients with repeated implantation failure capable of producing blastocysts in achieving pregnancies. |
| Databáze: | OpenAIRE |
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