Effects of the Glutamine Administration on T Helper Cell Regulation and Inflammatory Response in Obese Mice Complicated with Polymicrobial Sepsis
| Autor: | Sung Ling Yeh, Chiu Li Yeh, Der Yirng Hsieh, Ming-Tsan Lin, Po-Chu Lee, Hsueh Ju Wang, Li Han Su, Jin-Ming Wu, Po Da Chen, Po Jen Yang, Chun Chieh Huang |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
CD4-Positive T-Lymphocytes Male medicine.medical_specialty Article Subject medicine.medical_treatment T cell Glutamine Immunology Adipose tissue Mice Obese Inflammation Lung injury Proinflammatory cytokine Tight Junctions Sepsis 03 medical and health sciences Mice 0302 clinical medicine Adipokines Internal medicine medicine Pathology RB1-214 Animals Laparotomy business.industry Macrophages Body Weight 030208 emergency & critical care medicine Cell Biology T helper cell Lung Injury T-Lymphocytes Helper-Inducer medicine.disease Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Endocrinology Cytokine Adipose Tissue Cytokines medicine.symptom business Research Article |
| Zdroj: | Mediators of Inflammation Mediators of Inflammation, Vol 2020 (2020) |
| ISSN: | 1466-1861 0962-9351 |
| Popis: | This study investigated the impacts of GLN on inflammation and T cell dysregulation in obese mice complicated with sepsis. Mice were divided into normal control (NC) and high-fat diet groups. The high-fat diet provided 60% of energy from fat and was administered for 10 weeks to induce obesity. Mice fed with a high-fat diet were then assigned to sham (SH) and sepsis with saline (SS) or GLN (SG) groups. The SH group was subjected to laparotomy, while the sepsis group underwent cecal ligation and puncture (CLP). The SS group was intravenously injected with saline. The SG group was intravenously administered GLN after CLP. Mice were sacrificed at 12, 24, or 48 h post-CLP, respectively. Results demonstrated that in the presence of obesity, sepsis drove CD4+ T cells toward the helper T (Th)2 and Th17 lineages. Also, expressions of inflammatory cytokines and macrophage infiltration markers in adipose tissues and lungs were elevated. Treatment of obese mice with GLN after sepsis reversed Th polarization and downregulated macrophage infiltration and inflammatory cytokine, whereas the tight junction-associated protein expression increased in the lungs. These findings suggest that the intravenous administration of GLN to obese mice after sepsis modulated a more balanced Th cell lineage, alleviated inflammation, and attenuated lung injury. |
| Databáze: | OpenAIRE |
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