Using carbon nanotube probes for high-resolution three-dimensional imaging of cells
| Autor: | Jessica Koehne, Huan Yuan Chen, I-Chun Weng, R. M. Stevens, Tiffany Zink, Fu-Tong Liu, Zhao Deng, Gang-yu Liu |
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| Rok vydání: | 2010 |
| Předmět: |
Materials science
Atomic force microscopy Nanotubes Carbon High resolution Nanotechnology Carbon nanotube Membrane morphology Rat Basophilic Leukemia Microscopy Atomic Force Cell Membrane Structures Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials law.invention Rats Membrane Three dimensional imaging Imaging Three-Dimensional law Cell Line Tumor Microscopy Electron Scanning Animals Artifacts Instrumentation Filopodia |
| Zdroj: | Ultramicroscopy. 111(8) |
| ISSN: | 1879-2723 |
| Popis: | While atomic force microscopy (AFM) has become a promising tool for visualizing membrane morphology of cells, many studies have reported the presence of artifacts such as cliffs on the edges of cells. These artifacts shield important structural features such as lamellopodia, filopodia, microvilli and membrane ridges, which represent characteristic status in signaling processes such as spreading and activation. These cliff-like edges arise from a premature contact of the probe side contact with the cell prior to the probe top apex-cell contact. Carbon nanotube (CNT) modified AFM probes were utilized to address this drawback. Using rat basophilic leukemia (RBL) cells, this work revealed that CNT probes diminish cliff-like artifacts and enabled visualization of entire membrane morphology and structural features in three dimensions. The high aspect ratio of CNT probes provides a very effective remedy to the cliff-like artifacts as well as tip convolution of conventional probes, which shall enhance the validity and application of AFM in cellular biology research. |
| Databáze: | OpenAIRE |
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