Immunohistochemical expression of MAP1LC3A and MAP1LC3B protein in breast carcinoma tissues
| Autor: | Ekhlas Qaid Gazem Othman, Ahmad Faisal Mutee, Mei Lan Tan, Tengku Sifzizul Tengku Muhammad, Gurjeet Kaur |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Microbiology (medical) CA15-3 Pathology medicine.medical_specialty Adolescent Clinical Biochemistry CA 15-3 Breast Neoplasms Cell Count Adenocarcinoma Biology Immunoenzyme Techniques Young Adult Breast cancer Biomarkers Tumor medicine Humans Immunology and Allergy skin and connective tissue diseases Aged Neoplasm Staging Tissue microarray Carcinoma Ductal Breast Biochemistry (medical) Public Health Environmental and Occupational Health Cancer Original Articles Hematology Middle Aged medicine.disease Medical Laboratory Technology Carcinoma Intraductal Noninfiltrating Fluorescent Antibody Technique Direct Tissue Array Analysis Carcinoma Medullary Immunohistochemistry Female Breast carcinoma Microtubule-Associated Proteins MAP1LC3B |
| Zdroj: | Journal of Clinical Laboratory Analysis. 23:249-258 |
| ISSN: | 1098-2825 0887-8013 |
| DOI: | 10.1002/jcla.20309 |
| Popis: | Autophagy is a protein degradation process within the cell and its deregulation has been linked to various diseases and the formation of cancer. One of the important proteins involved in the autophagy process is microtubule‐associated protein 1 light chain 3 (MAP1LC3). The aims of this study were to determine the MAP1LC3A and MAP1LC3B protein expression in both normal and cancer breast tissues and to determine the relationship between the expression of these proteins and type of tissues. Immunohistochemistry assessments were carried out on tissue microarrays consisting of breast tissues. MAP1LC3A expression was detected in 52/56 of normal breast tissue cores and 65/67 of breast cancer tissue cores. MAP1LC3B expression was detected in 55/56 of normal breast tissue cores and 67/67 of breast cancer tissue cores. MAP1LC3A and MAP1LC3B protein are expressed in the majority of normal and cancer breast tissues. A large number of MAP1LC3A and MAP1LC3B positive breast cancer tissues cores have high proportion of stained cells (81–100%) as compared with normal breast tissues. However, a significantly higher number of breast cancer tissues were found to express the MAP1LC3A protein with strong immunoreactivity as compared with the normal tissues, suggesting that MAP1LC3A may play a role in breast cancer development. J. Clin. Lab. Anal. 23:249–258, 2009. © 2009 Wiley‐Liss, Inc. |
| Databáze: | OpenAIRE |
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