Novel expression of vascular cell adhesion molecule-1 (CD106) by squamous epithelium in experimental acute graft-versus-host disease
| Autor: | Brigette S. Adair, Diana Whitaker-Menezes, Judith C. Kim, Robert Korngold, Masatoshi Deguchi, George F. Murphy |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Pathology medicine.medical_specialty Immunoelectron microscopy Short Communication Graft vs Host Disease Vascular Cell Adhesion Molecule-1 Biology Pathology and Forensic Medicine Mice Immune system medicine Animals Receptor Cell adhesion Skin Mucous Membrane Cell adhesion molecule Mucous membrane Epithelial Cells Immunohistochemistry Epithelium Cell biology Transplantation Mice Inbred C57BL medicine.anatomical_structure |
| Zdroj: | The American journal of pathology. 161(3) |
| ISSN: | 0002-9440 |
| Popis: | Vascular cell adhesion molecule-1 (VCAM-1; CD106), the receptor for VLA-4, is an important mediator of adhesive and co-stimulatory interactions that govern cutaneous immune responses. Initial studies designed to elucidate temporal aspects of endothelial adhesion molecule induction in murine acute graft-versus-host disease (aGVHD) revealed unexpected and novel VCAM-1 expression by cutaneous and mucosal epithelial cells. Immunohistochemical techniques confirmed VCAM-1 staining as early as 7 days after transplantation in a distinctive subpopulation of squamous epithelial cells that normally occupy focal domains within the epidermal basal cell layer, the follicular infundibulum, and the dorsal lingual epithelium. Specifically, VCAM-1 expression was intimately associated with rete ridge-like prominences in footpad epidermis and in dorsal lingual epithelium. VCAM-1, as evaluated by serial section-labeling techniques, was preferentially expressed at sites of early epithelial infiltration by CD4(+) T cells. Western blot analysis confirmed expression of the 110-kd isoform of VCAM-1 in epithelium isolated from aGVHD animals, and immunoelectron microscopy demonstrated VCAM-1 reactivity restricted exclusively to epithelial cell plasma membranes. It is concluded that VCAM-1 is selectively expressed by discrete squamous epithelial subpopulations in murine aGVHD. As such, VCAM-1 may play a previously unrecognized role in mediating interactions between donor effector T lymphocytes and host epithelial cell targets. |
| Databáze: | OpenAIRE |
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