Expression of c-fos and c-jun mRNA following transient retinal ischemia: an approach using ligation of the retinal central artery in the rat
| Autor: | Shoichi Shimada, Masaya Tohyama, Yasuo Tang, Ichiro Ishimoto, Hiroyuki Morimura, Yasumasa Otori |
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| Rok vydání: | 1998 |
| Předmět: |
Male
Retinal Ganglion Cells Pathology medicine.medical_specialty Central retinal artery Proto-Oncogene Proteins c-jun Retinal Artery Optic Disk Ischemia Biology c-Fos Brain ischemia medicine.artery medicine Animals RNA Messenger Rats Wistar Ganglion cell layer Ligation In Situ Hybridization Retina Retinal Degeneration Anatomy medicine.disease Rats Ophthalmology medicine.anatomical_structure Reperfusion Injury Inner nuclear layer Nerve Degeneration Optic nerve biology.protein sense organs Proto-Oncogene Proteins c-fos |
| Zdroj: | Survey of ophthalmology. 42 |
| ISSN: | 0039-6257 |
| Popis: | The expression of the proto-oncogenes c-fos and c-jun was examined by in situ hybridization at various timepoints following transient retinal ischemia by means of ligation of the retinal central artery in the rat. Ischemia of 90-minute duration resulted in the degeneration of neurons in both the ganglion cell layer and the inner nuclear layer at 48 hours after reperfusion. The expression of c-fos and c-jun messenger RNA throughout the entire inner nuclear layer was transiently coinduced following 90-minute retinal ischemia with a peak at 1 hour after reperfusion. This expression was also found in the ganglion cell layer at 3 hours after reperfusion. Weak signals for c-fos and c-jun mRNA were observed at 24 hours after reperfusion and returned to near control levels by 48 hours. c-jun protein expression was detected in the ganglion cell layer, the middle of the inner nuclear layer, and optic nerve head at 3 hours, but not 1 hour, after lethal ischemia/reperfusion; however, c-fos protein expression was not detected after reperfusion. Whereas no neuronal degenerative changes were found at 7 days after 30-minute ischemic retina, c-fos and c-jun messenger RNA were also induced at 1 hour postreperfusion. To our knowledge, this study is the first report to show expression patterns of immediate-early genes after retinal ischemia/reperfusion. These results suggest that changes in expression of c-fos and c-jun after transient retinal ischemia are similar to those after transient brain ischemia, and the selective occlusion of the central retinal artery will provide a useful model for studying ischemic neuronal degeneration in vivo in the rat retina. |
| Databáze: | OpenAIRE |
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