Development of a Capillary Electrophoresis Method for the Separation of Fluoroquinolone Derivatives in Acidic Background Electrolyte
| Autor: | Árpád Gyéresi, Gabriel Hancu, Aura Rusu |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
separation
Analyte Chromatography business.industry Capillary action Electrolyte High-performance liquid chromatography acidic background electrolyte Electrophoresis chemistry.chemical_compound Capillary electrophoresis chemistry capillary zone electrophoresis Enoxacin Medicine fluoroquinolones General Pharmacology Toxicology and Pharmaceutics business General Dentistry Phosphoric acid medicine.drug |
| Zdroj: | Acta Medica Marisiensis, Vol 60, Iss 3, Pp 109-115 (2014) |
| ISSN: | 2247-6113 |
| Popis: | Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy, Tirgu Mureș, RomaniaIntroduction: Antibacterial quinolones class comprises a series of synthetic antibacterial agents, following the model of nalidixic acid. Be-cause of their common 6-fl uorosubtituent on the quinolone ring, fl uroquinolones are the most potent analogues with extended spectrum of activity and great pharmacokinetic properties. The applicability of capillary zone electrophoresis for the separation of fl uoro quinolones in acidic background electrolyte has been studied, our aim being the development of a capillary zone electrophoresis method for the simultaneous separation of six fl uoroquinolones and also to optimize the analytical conditions. The six studied fl uoroquinolones were ciprofl oxacin, enoxacin, enrofl oxacin, moxifl oxacin, ofl oxacin and sarafl oxacin. Material and methods: Preliminary, we studied the electrophoretic behavior of six fl uoroquinolones in an acidic pH, which highlighted the possibility of developing a separation method in this area of pH. Electrophoretic parameters infl uencing separation performance were studied and optimized. Results: A fast and reliable method has been developed, using a background electrolyte containing 100 mM phosphoric acid and the follow ing conditions: applied voltage: + 25 kV, temperature: 20°C, injection pressure 30 mbar – 5 sec, UV detection at 280 nm, capillary: 60 cm (52 cm effective length) x 50 μm, analyte concentration: 167 μg/ml. The separation of the studied fl uoroquinolones was achieved in les s than 8 minutes. Conclusions: Capillary zone electrophoresis using an acidic background electrolyte proved to be an effi cient tool in the separation of fl uo-roquinolones from different generations. Also the proposed methods are particular environment-friendly replacement and improvement of a common high performance liquid chromatography determination with rapid analysis time without using any organic solvents.Keywords: fl uoroquinolones, capillary zone electrophoresis, acidic background electrolyte, separationReceived: 11 June 2013 / Accepted: 7 June 2014 |
| Databáze: | OpenAIRE |
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