Mapping of avirulence genes in the rice blast fungus, Magnaporthe grisea, with RFLP and RAPD markers
Autor: | Marc J. Orbach, Jean Loup Notteghem, Waly Dioh, Marc-Henri Lebrun, Didier Tharreau |
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Jazyk: | angličtina |
Rok vydání: | 2000 |
Předmět: |
Genetic Markers
Positional cloning Physiology Pouvoir pathogène Genes Fungal Molecular Sequence Data Gene mapping RAPD Magnaporthe grisea Marqueur génétique Amino Acid Sequence DNA Fungal DNA Primers Plant Diseases H20 - Maladies des plantes Genetics biology Base Sequence Virulence Fungal genetics Bulked segregant analysis Chromosome Mapping Oryza General Medicine Maladie fongique biology.organism_classification Random Amplified Polymorphic DNA Technique Magnaporthe Genetic marker Gène Carte génétique RFLP Restriction fragment length polymorphism Agronomy and Crop Science Polymorphism Restriction Fragment Length |
Zdroj: | Molecular Plant-Microbe Interactions |
Popis: | Three genetically independent avirulence genes, AVR1-Irat7, AVR1-MedNoï, and AVR1-Ku86, were identified in a cross involving isolates Guy11 and 2/0/3 of the rice blast fungus, Magnaporthe grisea. Using 76 random progeny, we constructed a partial genetic map with restriction fragment length polymorphism (RFLP) markers revealed by probes such as the repeated sequences MGL/MGR583 and Pot3/MGR586, cosmids from the M. grisea genetic map, and a telomere sequence oligonucleotide. Avirulence genes AVR1-MedNoï and AVR1-Ku86 were closely linked to te-lomere RFLPs such as marker TelG (6 cM from AVR1-MedNoï) and TelF (4.5 cM from AVR1-Ku86). Avirulence gene AVR1-Irat7 was linked to a cosmid RFLP located on chromosome 1 and mapped at 20 cM from the avirulence gene AVR1-CO39. Using bulked segregant analysis, we identified 11 random amplified polymorphic DNA (RAPD) markers closely linked (0 to 10 cM) to the avirulence genes segregating in this cross. Most of these RAPD markers corresponded to junction fragments between known or new transposons and a single-copy sequence. Such junctions or the whole sequences of single-copy RAPD markers were frequently absent in one parental isolate. Single-copy sequences from RAPD markers tightly linked to avirulence genes will be used for positional cloning. |
Databáze: | OpenAIRE |
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