| Autor: |
S. Kaufman, Antoine Vercruysse, Serge Waterschoot, Vera Rogiers, Karin Mertens |
| Rok vydání: |
1996 |
| Předmět: |
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| Zdroj: |
Toxicology in Vitro. 10:507-511 |
| ISSN: |
0887-2333 |
| DOI: |
10.1016/0887-2333(96)82584-7 |
| Popis: |
The production of thiobarbituric acid reactive substances (TBARS) and the content of reduced glutathione (GSH) were determined in primary monolayer cultures of adult rat hepatocytes and in their co-cultures with rat epithelial cells, both exposed for 90 min to 2 mm tertiary butylhydroperoxide. Under these stress-induced conditions, TBARS production tended to be less increased in co-cultured cells than in conventionally cultured hepatocytes. A total depletion of GSH content was measured in conventional cultures from the third culture day, whereas in co-cultures a decrease in GSH content was observed until the third day of culture, followed by a low but constant GSH level that was maintained until the end of the culture period. Oxidative stress appears to be less pronounced in co-cultures than in conventional cultures; the former cope better with externally induced oxidative stress than do the latter. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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