Ultra-photostable, non-cytotoxic, and highly fluorescent quantum nanospheres for long-term, high-specificity cell imaging
| Autor: | Liman Sai, Yuanyuan Su, Lianhui Wang, Haoting Lu, Yao He, Mei Hu, Chunhai（樊春海） Fan |
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| Rok vydání: | 2011 |
| Předmět: |
Materials science
Light Biocompatibility Cell Biophysics Bioengineering Nanotechnology Photochemistry Fluorescence Biomaterials In vivo Quantum Dots medicine Animals Humans Irradiation Cytotoxicity Quantum Fluorescent Dyes Molecular Imaging HEK293 Cells medicine.anatomical_structure Mechanics of Materials Quantum dot Ceramics and Composites K562 Cells Nanospheres |
| Zdroj: | Biomaterials. 32:2133-2140 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2010.11.034 |
| Popis: | A new class of fluorescent quantum nanospheres (QNs) is directly achieved in aqueous phase through a facile one-pot microwave irradiation (MWI) strategy. Multi-color QNs with maximum emission wavelengths ranging from 525 to 610 nm and PLQY of 30-60% are facilely prepared through this new MWI strategy. In addition to strong fluorescence, these QNs possess excellent photostability, preserving ∼90% of the original intensity after 70 min high-power UV irradiation (100 W Xeon lamp). In sharp contrast, the fluorescence of CdTe/CdS/ZnS core-shell-shell quantum dots (QDs), recognized as established fluorescent probes with robust photostability, decrease to ∼50% under the same conditions. Besides, cytotoxicity assessment demonstrates that the prepared QNs exhibit favorable cytocompatibility to K562 cells with high concentration (3 μmol) and long-time incubation (24 h). Furthermore, cellular imaging results demonstrate that the as-prepared QNs are remarkably efficacious for long-term and high-specificity immunofluorescent cellular labeling, and multi-color cell imaging. Our systematical investigation clearly shows that these high-performance QNs may serve as practical and powerful tools for various biological researches, such as in vivo and in vitro imaging. |
| Databáze: | OpenAIRE |
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