Brain Morphological Changes in COVID-19
Autor: | Artem Kuzovlev, Alexei V. Volkov, Irina Ostrova, Arkady Golubev, Anastasia S. Babkina |
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Jazyk: | ruština |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Karyolysis Retrograde Degeneration Pathology medicine.medical_specialty brain H&E stain Autopsy Brain damage Critical Care and Intensive Care Medicine 03 medical and health sciences symbols.namesake 0302 clinical medicine morphology medicine ischemic stroke neun neurotropic action of sars-cov-2 virus business.industry Cerebral infarction RC86-88.9 Medical emergencies. Critical care. Intensive care. First aid medicine.disease 030104 developmental biology nervous system covid-19 Nissl body symbols Histopathology medicine.symptom business 030217 neurology & neurosurgery |
Zdroj: | Obŝaâ Reanimatologiâ, Vol 17, Iss 3, Pp 4-15 (2021) |
ISSN: | 2411-7110 1813-9779 |
Popis: | The aim of the study was to identify the pathomorphology of brain damage in patients who died of COVID-19.Material and methods. Autopsy reports and autopsy brain material of 17 deceased patients with pre-mortem confirmed COVID-19 infection were analyzed. Fatal cases in which COVID19 was the major cause of death were included in the study. Five people were diagnosed with cerebral infarction. Organ samples were taken for histological examination during autopsy. Sections were stained with hematoxylin and eosin and by Nissl to assess brain histopathology. To study the vascular basal membranes the PAS reaction was used, to detect fibrin in vessels phosphotungstic acid-hematoxylin (PTAH) staining was used, to determine DNA in nuclei sections were stained according to Feulgen, to detect RNA in neuronal nuclei and cytoplasm sections were stained with methyl green-pyronin. Immunohistochemical study of a neuronal marker, nuclear protein NeuN, was performed to assess neuronal damage.Results. The signs of neuronal damage found in patients who died of COVID-19 included nonspecific changes of nerve cells (acute swelling, retrograde degeneration, karyolysis and cytolysis, ‘ghost' cells, neuronophagia and satellitosis) and signs of circulatory disorders (perivascular and pericellular edema, diapedesis, congested and engorged microvasculature).Conclusion. Brain histopathological data indicate damage to the central nervous system in COVID-19 patients. Ischemic stroke in patients with COVID-19 is mostly caused by a combination of hypoxia resulting from respiratory failure and individual risk factors, including cerebrovascular atherosclerosis and hypertension. |
Databáze: | OpenAIRE |
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