Enantiomer discrimination in β-phenylalanine degradation by a newly isolated Paraburkholderia strain BS115 and type strain PsJN

Autor: Buß, Oliver, Dold, Sarah-Marie, Obermeier, Pascal, Litty, Dennis, Muller, Delphine, Grüninger, Jens, Rudat, Jens
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: AMB Express
AMB Express, Vol 8, Iss 1, Pp 1-13 (2018)
AMB express, 8 (1), Article: 149
ISSN: 2191-0855
Popis: Despite their key role in numerous natural compounds, β-amino acids have rarely been studied as substrates for microbial degradation. Fermentation of the newly isolated Paraburkholderia strain BS115 and the type strain P. phytofirmans PsJN with β-phenylalanine (β-PA) as sole nitrogen source revealed (S)-selective transamination of β-PA to the corresponding β-keto acid by both strains, accompanied by substantial formation of acetophenone (AP) from spontaneous decarboxylation of the emerging β-keto acid. While the PsJN culture became stationary after entire (S)-β-PA consumption, BS115 showed further growth at a considerably slower rate, consuming (R)-β-PA without generation of AP which points to a different degradation mechanism for this enantiomer. This is the first report on degradation of both enantiomers of any β-amino acid by one single bacterial strain. Electronic supplementary material The online version of this article (10.1186/s13568-018-0676-2) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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