Expression of Cholera Toxin B Subunit in Transgenic Rice Endosperm
| Autor: | Tae Geum Kim, Youn-Soo Cha, Sándor Tömösközi, Moon Sik Yang, Mária Oszvald, László Tamás, Barnabas Jenes, Tae-Jin Kang |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Cholera Toxin
DNA Plant Protein subunit Blotting Western Genetic Vectors Enzyme-Linked Immunosorbent Assay Receptors Cell Surface Bioengineering Genetically modified crops Biology complex mixtures Applied Microbiology and Biotechnology Biochemistry Endosperm law.invention Gene Expression Regulation Plant law Molecular Biology Gene Expression vector fungi food and beverages Oryza Blotting Northern Plants Genetically Modified Molecular biology Genetically modified rice Transformation (genetics) Seeds embryonic structures Recombinant DNA Biotechnology |
| Zdroj: | Molecular Biotechnology. 40:261-268 |
| ISSN: | 1559-0305 1073-6085 |
| DOI: | 10.1007/s12033-008-9083-2 |
| Popis: | The synthetic cholera toxin B subunit (CTB) gene, modified according to the optimized codon usage of plant genes, was introduced into a plant expression vector and expressed under the control of the Bx17 HMW (high molecular weight) wheat endosperm-specific promoter containing an intron of the rice act1. The recombinant vector was transformed into rice plants using a biolistic-mediated transformation method. Stable integration of the synthetic CTB gene into the chromosomal DNA was confirmed by PCR amplification analysis. A high level of CTB (2.1% of total soluble protein) was expressed in the endosperm tissue of the transgenic rice plants. The synthetic CTB produced only in the rice endosperm demonstrated strong affinity for G(M1)-ganglioside, thereby suggesting that the CTB subunits formed an active pentamer. The successful expression of CTB genes in transgenic plants makes it a powerful tool for the development of a plant-derived edible vaccine. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink