Cloning and functional analysis of a new laccase gene from Trametes sp. 48424 which had the high yield of laccase and strong ability for decolorizing different dyes
| Autor: | Rui Zhuo, Xiaoyu Zhang, Xia Wan, Fangfang Fan, Yang Yang, Mulan Jiang, Su Sun |
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| Rok vydání: | 2011 |
| Předmět: |
Environmental Engineering
Color Bioengineering Biology Protein Engineering Pichia pastoris Complementary DNA Enzyme Stability Gene expression Coloring Agents Waste Management and Disposal Trametes Laccase chemistry.chemical_classification Renewable Energy Sustainability and the Environment Genetic transfer General Medicine biology.organism_classification Molecular biology Yeast Amino acid Enzyme Activation Biochemistry chemistry Heterologous expression |
| Zdroj: | Bioresource Technology. 102:3126-3137 |
| ISSN: | 0960-8524 |
| Popis: | The laccase gene lac48424-1 and its corresponding full-length cDNA were cloned and characterized from a novel white-rot fungi Trametes sp. 48424 which had the high yield of laccase and strong ability for decolorizing different dyes. The 1563 bp full-length cDNA of lac48424-1 encoded a mature laccase protein containing 499 amino acids preceded by a signal peptide of 21 amino acids. The deduced protein sequence of LAC48424-1 showed high similarity with other known fungal laccases and contained four copper-binding conserved domains of typical laccase protein. The functionality of lac48424-1 gene encoding active laccase was verified by expressing the gene in the yeast Pichia pastoris successfully. It was found that the recombinant laccase produced by the yeast transformant could decolorize different dyes. The 5'-flanking sequence upstream of start codon was obtained by Self-Formed Adaptor PCR. Many putative cis-acting responsive elements involved in the transcriptional regulation were identified in the promoter region of lac48424-1. |
| Databáze: | OpenAIRE |
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