Development and Characterization of Polymorphic Microsatellite Loci in Phellodendron amurense (Rutaceae)
| Autor: | Shijie Han, Yuangang Zu, Xiao-rui Guo, Jinghua Yu, Shu-Sheng Yuan, Chun-Jing Wang, Chang-Mei Chen |
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| Rok vydání: | 2013 |
| Předmět: |
Genetics
education.field_of_study Genetic diversity biology Phellodendron amurense Population microsatellite marker Locus (genetics) Plant Science Primer Note biology.organism_classification lcsh:QK1-989 simple sequence repeat markers lcsh:Biology (General) lcsh:Botany Genetic structure Microsatellite Genomic library Allele genetic conservation education lcsh:QH301-705.5 Rutaceae Ecology Evolution Behavior and Systematics |
| Zdroj: | Applications in Plant Sciences Applications in Plant Sciences, Vol 1, Iss 3, p 1200321 (2013) |
| ISSN: | 2168-0450 |
| Popis: | Premise of the study: Microsatellite markers were developed for the rare species Phellodendron amurense to assess the genetic diversity and population structure of this plant. Methods and Results: In total, 27 microsatellite markers were developed for P. amurense by using an enriched genomic library and hybridization; all of these primers successfully amplified DNA fragments in P. amurense. These markers were screened in 74 individuals from four populations in China; 15 loci were found to be polymorphic, with the number of alleles per locus ranging from one to nine. Conclusions: The microsatellite markers developed here represent a useful tool for studying the population genetic structure of P. amurense and to inform toward the development of effective conservation programs for this species. |
| Databáze: | OpenAIRE |
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