Exposure of Metal Oxide Nanoparticles on the Bioluminescence Process of Pu- and Pm-lux Recombinant P. putida mt-2 Strains
| Autor: | Robert S. Burlage, Dong-Chan Koh, In Chul Kong, Kyung-Seok Ko, Sohyeon Lee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
inorganic chemicals
General Chemical Engineering Nanoparticle Article law.invention law mental disorders Bioluminescence General Materials Science Inducer Pm- and Pu-lux recombinant inducer QD1-999 health care economics and organizations EC50 Strain (chemistry) biology Chemistry Non-blocking I/O technology industry and agriculture respiratory system biology.organism_classification bioluminescence Pseudomonas putida Recombinant DNA nanoparticles Nuclear chemistry |
| Zdroj: | Nanomaterials, Vol 11, Iss 2822, p 2822 (2021) Nanomaterials Volume 11 Issue 11 |
| ISSN: | 2079-4991 |
| Popis: | Comparison of the effects of metal oxide nanoparticles (NPs CuO, NiO, ZnO, TiO2, and Al2O3) on different bioluminescence processes was evaluated using two recombinant (Pm-lux and Pu-lux) strains of Pseudomonas putida mt-2 with same inducer exposure. Different sensitivities and responses were observed according to the type of NPs and recombinant strains. EC50 values were determined. The negative effects on the bioluminescence activity of the Pm-lux strain was greater than for the Pu-lux strains for all NPs tested. EC50 values for the Pm-lux strain were 1.7- to 6.2-fold lower (corresponding to high inhibition) than for Pu-lux. ZnO NP caused the greatest inhibition among the tested NPs in both strains, showing approximately 11 times less EC50s of CuO, which appeared as the least inhibited. Although NPs showed different sensitivities depending on the bioluminescence process, similar orders of EC50s for both strains were observed as follows: ZnO > NiO, Al2O3 > TiO2 > CuO. More detailed in-depth systematic approaches, including in the field of molecular mechanisms, is needed to evaluate the accurate effect mechanisms involved in both bioluminescence metabolic processes. |
| Databáze: | OpenAIRE |
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