High-level fed-batch fermentative expression of an engineered Staphylococcal protein A based ligand in E. coli: purification and characterization
| Autor: | Martin Kangwa, Ayse Nur Polat, Mariano Grasselli, Marcelo Fernández-Lahore, Kanaka Durga Devi Gorrepati, Vikas Yelemane |
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| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
Biophysics Biomass Industrial fermentation INGENIERÍAS Y TECNOLOGÍAS Biology medicine.disease_cause FED-BATCH FERMENTATION 01 natural sciences Applied Microbiology and Biotechnology Biotecnología Industrial Fed-batch fermentation 03 medical and health sciences Laboratory flask 010608 biotechnology medicine High cell density Food science Escherichia coli 030304 developmental biology Gram 2. Zero hunger 0303 health sciences RECOMBINANT PROTEIN A purl.org/becyt/ford/2.9 [https] E. coli Ligand (biochemistry) AVIPURE AviPure purl.org/becyt/ford/2 [https] Biochemistry Yield (chemistry) Recombinant protein A E. COLI HIGH CELL DENSITY Original Article Fermentation |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET AMB Express |
| ISSN: | 2191-0855 |
| Popis: | The major platform for high level recombinant protein production is based on genetically modified microorganisms like Escherichia coli (E. coli) due to its short dividing time, ability to use inexpensive substrates and additionally, its genetics is comparatively simple, well characterized and can be manipulated easily. Here, we investigated the possibilities of finding the best media for high cell density fermentation, by analyzing different media samples, focusing on improving fermentation techniques and recombinant protein production. Initial fermentation of E. coli BL21 DE3:pAV01 in baffled flasks showed that high cell density was achieved when using complex media, Luria–Bertani (LB) and Terrific medium broth (TB) (10 and 14 g/L wet weight, respectively), as compared to mineral media M9, modified minimal medium (MMM) and Riesenberg mineral medium (RM) (7, 8 and 7 g/L, respectively). However, in fed-batch fermentation processes when using MMM after 25 h cultivation, it was possible to yield an optical density (OD600) of 139 corresponding to 172 g/L of wet biomass was produced in a 30 L TV Techfors-S Infors HT fermenter, with a computer controlled nutrient supply (glucose as a carbon source) delivery system, indicating nearly 1.5 times that obtained from TB. Upon purification, a total of 1.65 mg/g of protein per gram cell biomass was obtained and the purified AviPure showed affinity for immunoglobulin. High cell density fed batch fermentation was achieved by selecting the best media and growth conditions, by utilizing a number of fermentation parameters like media, fermentation conditions, chemical concentrations, pO2 level, stirrer speed, pH level and feed media addition. It is possible to reach cell densities higher than shake flasks and stirred tank reactors with the improved oxygen transfer rate and feed. Fil: Kangwa, Martin. Jacobs University; Alemania Fil: Yelemane, Vikas. Jacobs University; Alemania Fil: Polat, Ayse Nur. Jacobs University; Alemania Fil: Gorrepati, Kanaka Durga Devi. Jacobs University; Alemania Fil: Grasselli, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina Fil: Fernández Lahore, Marcelo. Jacobs University; Alemania |
| Databáze: | OpenAIRE |
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