Potential Mechanism of Action of 3′-Demethoxy-6-O-demethyl-isoguaiacin on Methicillin Resistant Staphylococcus aureus
| Autor: | Isaías Balderas-Rentería, María del Rayo Camacho-Corona, Juan Manuel J. Favela-Hernández, Elvira Garza-González, Aldo F. Clemente-Soto |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Pharmaceutical Science
Microbial Sensitivity Tests Naphthols methicillin-resistant Staphylococcus aureus Real-Time Polymerase Chain Reaction medicine.disease_cause Article Lignans Analytical Chemistry Microbiology law.invention lcsh:QD241-441 Bacterial Proteins mode of action lcsh:Organic chemistry law Drug Discovery medicine Larrea tridentata Physical and Theoretical Chemistry Mode of action microarrays 3′-demethoxy-6-O-demethylisoguaiacin Polymerase chain reaction Oligonucleotide Array Sequence Analysis biology Gene Expression Profiling Cell Membrane Organic Chemistry Gene Expression Regulation Bacterial biology.organism_classification Methicillin-resistant Staphylococcus aureus Larrea Anti-Bacterial Agents Plant Leaves real time PCR Real-time polymerase chain reaction Chemistry (miscellaneous) Staphylococcus aureus Gene chip analysis Molecular Medicine ATP-Binding Cassette Transporters Antibacterial activity Bacteria |
| Zdroj: | Molecules, Vol 20, Iss 7, Pp 12450-12458 (2015) Molecules Volume 20 Issue 7 Pages 12450-12458 |
| ISSN: | 1420-3049 |
| Popis: | Bacterial infections represent one of the main threats to global public health. One of the major causative agents associated with high morbidity and mortality infections in hospitals worldwide is methicillin-resistant Staphylococcus aureus. Therefore, there is a need to develop new antibacterial agents to treat these infections, and natural products are a rich source of them. In previous studies, we reported that lignan 3′-demethoxy-6-O-demethylisoguaiacin, isolated and characterized from Larrea tridentate, showed the best activity towards methicillin-resistant S. aureus. Thus, the aim of this study was to determine the potential molecular mechanism of the antibacterial activity of 3′-demethoxy-6-O-demethylisoguaiacin against methicillin-resistant S. aureus using microarray technology. Results of microarray genome expression were validated by real-time polymerase chain reaction (RT-PCR). The genetic profile expression results showed that lignan 3′-demethoxy-6-O-demethylisoguaiacin had activity on cell membrane affecting proteins of the ATP-binding cassette (ABC) transport system causing bacteria death. This molecular mechanism is not present in any antibacterial commercial drug and could be a new target for the development of novel antibacterial agents. |
| Databáze: | OpenAIRE |
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