Broad spectrum anti-RNA virus activities of titanium and vanadium substituted polyoxotungstates
Autor: | Junko Kodama, Eiichi Kodama, Toshihiro Yamase, Shuichi Mori, Shibro Shigeta, Kazuo Takahashi |
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Rok vydání: | 2003 |
Předmět: |
Paramyxoviridae
Polymers Stereochemistry Orthomyxoviridae Vanadium chemistry.chemical_element Microbial Sensitivity Tests Virus Replication Antiviral Agents Virus Cell Line Parainfluenza virus type 2 Virology Animals Humans RNA Viruses Mononegavirales Titanium Pharmacology biology Biological activity Tungsten Compounds biology.organism_classification Flavivirus chemistry HIV-1 |
Zdroj: | Antiviral Research. 58:265-271 |
ISSN: | 0166-3542 |
DOI: | 10.1016/s0166-3542(03)00009-3 |
Popis: | Seven polyoxotungstates substituted with vanadium or titanium atoms were examined for their activity against Flaviviridae (Dengue fever virus, DFV), Orthomyxoviridae (influenza virus type A, fluV-A), Paramyxoviridae (respiratory syncytial virus, RSV, parainfluenza virus type 2, PfluV-2 and canine distemper virus, CDV) and Lentiviridae (human immunodeficiency virus type 1, HIV-1) families. Among the seven polyoxotungstates examined, PM-43 [K(5)[SiVW(11)O(40)]], PM-47 [K(7)[BVW(11)O(40)]], and PM-1001 [K(10)Na(VO)(3)(SbW(9)O(33))(2)]26H(2)O contained vanadium. PM-1002 had the same core structure of (VO)(3)(SbW(9)O(33))(2) as PM-1001; however, three V atoms of PM-1001 consisted of two V(IV) and one V(V) and those of PM-1002 consisted of three V(IV). On the other hand, PM-518 [[Et(2)NH(2)](7)[PTi(2)W(10)O(40)]], PM-520 [Pri(2)NH(2)](5)[PTiW(11)O(40)] and PM-523 [PriNH(3)](6)H[PTi(2)W(10)O(38)(O(2))(2)]H(2)O all contained titanium. All compounds showed broad spectrum antiviral activity against all viruses examined except for PMs-43, -518 and -523 which did not exhibit inhibitory activity at >/=50 microM against PfluV-2, CDV and DFV, respectively. All compounds were inhibitory against HIV replication at an EC(50) of less than 2.0 microM. Among them, PMs-1001 and -1002 showed the most potent inhibition. The compounds were not toxic for MDCK, HEp-2 and Vero cells at a concentration of 200 microM. For the exponentially growing MT-4 cells, the vanadium containing polyoxometalates (PMs-43, 47, 1001, 1002) showed toxicity at concentrations between 41 and 47 microM. On the other hand, titanium containing polyoxometalates (PMs-518, -520, -523) were not toxic at 100 microM. The mechanism of anti-HIV action of PM-1001 was analyzed: it affected the binding of HIV to the cell membrane and syncytium formation between HIV-infected and uninfected cells. |
Databáze: | OpenAIRE |
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