Translation, modification and cellular distribution of two AC4 variants of African cassava mosaic virus in yeast and their pathogenic potential in plants
| Autor: | Holger Jeske, Jens Pfannstiel, Peter Rau, Benjamin Schäfer, Katharina Hipp |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Recombinant Fusion Proteins Gene Expression Nicotiana benthamiana Genome Viral Green fluorescent protein Viral Proteins 03 medical and health sciences African cassava mosaic virus Start codon Genes Reporter Yeasts Virology Schizosaccharomyces Amino Acid Sequence Gene Plant Diseases Myristoylation Genetics Microscopy Confocal biology Begomovirus food and beverages biology.organism_classification Protein Transport Open reading frame Phenotype 030104 developmental biology Protein Biosynthesis |
| Zdroj: | Virology. 498:136-148 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2016.07.011 |
| Popis: | Plant infecting geminiviruses encode a small (A)C4 protein within the open reading frame of the replication-initiator protein. In African cassava mosaic virus, two in-frame start codons may be used for the translation of a longer and a shorter AC4 variant. Both were fused to green fluorescent protein or glutathione-S-transferase genes and expressed in fission yeast. The longer variant accumulated in discrete spots in the cytoplasm, whereas the shorter variant localized to the plasma membrane. A similar expression pattern was found in plants. A myristoylation motif may promote a targeting of the shorter variant to the plasma membrane. Mass spectrometry analysis of the yeast-expressed shorter variant detected the corresponding myristoylation. The biological relevance of the second start codon was confirmed using mutated infectious clones. Whereas mutating the first start codon had no effect on the infectivity in Nicotiana benthamiana plants, the second start codon proved to be essential. |
| Databáze: | OpenAIRE |
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