Investigation of genetic variants of α-1 acid glycoprotein by ultra-performance liquid chromatography–mass spectrometry
| Autor: | Tibor Kremmer, László Drahos, Judit Krenyacz, Lívia Budai, Oliver Ozohanics, Károly Vékey, Krisztina Ludányi |
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| Rok vydání: | 2008 |
| Předmět: |
chemistry.chemical_classification
biology Melanoma Genetic Variation Reproducibility of Results Cancer Orosomucoid medicine.disease Biochemistry Molecular biology Mass Spectrometry Analytical Chemistry Lymphoma chemistry Liquid chromatography–mass spectrometry medicine biology.protein Humans Glycoprotein Ovarian cancer Gene Chromatography High Pressure Liquid |
| Zdroj: | Analytical and Bioanalytical Chemistry. 393:991-998 |
| ISSN: | 1618-2650 1618-2642 |
| Popis: | Genetic variants of human plasma alpha-1 acid glycoprotein (AGP) have been studied in cancer, compared with a group of healthy control. AGP has four genetic variants: AGP F1, F2, and S variants correspond to the ORM1 gene whereas AGP A corresponds to the ORM2 gene. The proportion of ORM1 and ORM2 variants were studied in plasma using a novel UPLC-MS method. Plasma total AGP level was 0.5 +/- 0.2 g L(-1) and the proportions of the ORM1 and ORM2 variants were 76.3 +/- 8.2% and 23.7 +/- 8.2%, respectively. In cancer plasma AGP levels increased fourfold and the proportion of ORM1 variants increased to 88.7 +/- 6.8%. Changes in the proportion of genetic variants due to cancer were clearly significant, as shown by statistical analysis. Three different cancer types have been studied, lymphoma, melanoma, and ovarian cancer. The results did not show any difference depending on cancer type. The results indicate that, in accordance with prior expectations, the ORM1 variant is predominantly responsible for the acute-phase property of AGP. |
| Databáze: | OpenAIRE |
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