How long can culturable bacteria and total DNA persist in environmental waters? The role of sunlight and solid particles
| Autor: | Dolores Gutiérrez-Cacciabue, Verónica Beatriz Rajal, Alicia Graciela Cid |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
DNA Bacterial Environmental Engineering SOLID PARTICLES 030106 microbiology Fresh Water INGENIERÍAS Y TECNOLOGÍAS 010501 environmental sciences 01 natural sciences 03 medical and health sciences BACTERIAL INDICATORS Botany Environmental Chemistry Seawater CULTURE-BASED METHODS ENVIRONMENTAL WATERS Ingeniería del Medio Ambiente Waste Management and Disposal 0105 earth and related environmental sciences biology Solid particle Bacteria Ecology biology.organism_classification Pollution QPCR Sunlight SUNLIGHT INACTIVATION Ingeniería Medioambiental y Geológica Geotécnicas Water Microbiology Environmental Monitoring |
| Popis: | In this work, sunlight inactivation of two indicator bacteria in freshwater, with and without solid particles, was studied and the persistence of culturable cells and total DNA was compared. Environmental water was used to prepare two matrices, with and without solid particles, which were spiked with Escherichia coli and Enterococcus faecalis. These matrices were used to prepare microcosm bags that were placed in two containers: one exposed to sunlight and the other in the dark. During one month, samples were removed from each container and detection was done by membrane filter technique and real-time PCR. Kinetic parameters were calculated to assess sunlight effect. Indicator bacteria without solid particles exposed to sunlight suffered an immediate decay (40h in the dark with particles). In this case active cells were more prone to sunlight than total DNA and the protective effect of solid particles was also observed. Results highlight that the effects caused by the parameters which describe the behavior of culturable microorganisms and total DNA in water are different and must be included in simulation models but without forgetting that these parameters will also depend on bacterial properties, sensitizers, composition, type, and uses of the aquatic environment under assessment. Fil: Gutiérrez Cacciabue, Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina Fil: Cid, Alicia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina Fil: Rajal, Verónica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina. Nanyang Technological University; Singapur |
| Databáze: | OpenAIRE |
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