Hollow magnetic-fluorescent nanoparticles for dual-modality virus detection
| Autor: | Akhilesh Babu Ganganboina, Indra Memdi Khoris, Fuyuki Abe, Toshimi Hara, Enoch Y. Park, Ruey-an Doong, Tetsuro Suzuki, Ankan Dutta Chowdhury, Tian-Cheng Li |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Dual-modality
Materials science Biomedical Engineering Biophysics Magnetic separation Oxide Nanoparticle Nanotechnology 02 engineering and technology Biosensing Techniques 01 natural sciences Fluorescence Nanomaterials chemistry.chemical_compound Magnetics Virus diagnostics Quantum Dots Electrochemistry Multicomponent nanoparticles Fluorescent Dyes Bio-separation Nanocomposite Quenching (fluorescence) 010401 analytical chemistry Impedance General Medicine equipment and supplies 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Quantum dot Nanoparticles 0210 nano-technology Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 170:112680 |
| ISSN: | 0956-5663 |
| Popis: | Combination of magnetic nanomaterials with multifunctionality is an emerging class of materials that exhibit tremendous potential in advanced applications. Synthesizing such novel nanocomposites without compromising magnetic behavior and introducing added functional properties is proven challenging. In this study, an optically active quantum dot (QD) (core) encapsulated inside iron oxide (hollow shell) is prepared as the first electrochemical/fluorescence dual-modality probe. Presence of magnetic layer on the surface enables excellent magnetic property and the encapsulating of QDs on the hollow shell structure maintains the fluorescence with minimal quenching effect, endowing for potential application with fluorescence modality readout. We successfully demonstrate dual-modality sensing utilizing of QD-encapsulated magnetic hollow sphere nanoparticles (QD@MHS NPs) with magnetic separation ability and highly integrated multimodal sensing for the detection of various viruses including hepatitis E virus (HEV), HEV-like particles (HEV-LPs), norovirus-like particles (NoV-LPs), and norovirus (NoV) from clinical specimens. Most importantly, fecal samples of HEV-infected monkey are successfully diagnosed with sensitivity similar to gold standard real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR). This well-defined QD@MHS NPs-based nanoplatform intelligently integrates dual-modality sensing and magnetic bio-separation, which open a gateway to provide an efficient point-of care testing for virus diagnostics. |
| Databáze: | OpenAIRE |
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