Alliumtelomeres unmasked: the unusual telomeric sequence (CTCGGTTATGGG)nis synthesized by telomerase
| Autor: | Petr Fajkus, Zdeňka Sitová, Jiří Fajkus, Eva Sýkorová, Roman Gogela, Jan Hapala, Martina Dvořáčková, Vratislav Peška, Jana Fulnečková |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Telomerase Protein subunit Plant Science Biology 01 natural sciences Asparagales Chromosomes Plant Allium Restriction fragment Evolution Molecular 03 medical and health sciences Genetics Telomerase reverse transcriptase In Situ Hybridization Fluorescence Phylogeny Base Sequence Phylogenetic tree Computational Biology food and beverages Genomics Sequence Analysis DNA Cell Biology Telomere biology.organism_classification 030104 developmental biology biology.protein Transcriptome 010606 plant biology & botany |
| Zdroj: | The Plant Journal. 85:337-347 |
| ISSN: | 0960-7412 |
| DOI: | 10.1111/tpj.13115 |
| Popis: | Phylogenetic divergence in Asparagales plants is associated with switches in telomere sequences. The last switch occurred with divergence of the genus Allium (Amaryllidaceae) from the other Allioideae (formerly Alliaceae) genera, resulting in uncharacterized telomeres maintained by an unknown mechanism. To characterize the unknown Allium telomeres, we applied a combination of bioinformatic processing of transcriptomic and genomic data with standard approaches in telomere biology such as BAL31 sensitivity tests, terminal restriction fragment analysis, the telomere repeat amplification protocol (TRAP), and fluorescence in situ hybridization (FISH). Using these methods, we characterize the unusual telomeric sequence (CTCGGTTATGGG)n present in Allium species, demonstrate its synthesis by telomerase, and characterize the telomerase reverse transcriptase (TERT) subunit of Allium cepa. Our findings open up the possibility of studying the molecular details of the evolutionary genetic change in Allium telomeres and its possible role in speciation. Experimental studies addressing the implications of this change in terms of the interplay of telomere components may now be designed to shed more light on telomere functions and evolution in general. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text