Cytoskeletal features of alveolar myofibroblasts and pericytes in normal human and rat lung

Autor: C. Ribaux, Christine Chaponnier, Giulio Gabbiani, Yusuf Kapanci
Rok vydání: 1992
Předmět:
Pathology
medicine.medical_specialty
Histology
Stromal cell
Muscle
Smooth
Vascular/cytology/ ultrastructure
Fluorescent Antibody Technique
Vimentin
macromolecular substances
Fibroblasts/cytology/ ultrastructure
ddc:616.07
Antibodies
Muscle
Smooth
Vascular
Desmin
Calcium-Binding Proteins/analysis/immunology
Pulmonary Alveoli/cytology/ ultrastructure
Arterioles/cytology
Cytoskeleton/chemistry/ ultrastructure
medicine
Animals
Humans
Antibodies/immunology
Cytoskeleton
Microscopy
Immunoelectron
Lung
Gelsolin
Lung/cytology/ ultrastructure
biology
Desmin/analysis/immunology
Calcium-Binding Proteins
Microfilament Proteins
Vimentin/analysis/immunology
respiratory system
Fibroblasts
Actins
Rats
Microfilament Proteins/analysis/immunology
Pulmonary Alveoli
Arterioles
medicine.anatomical_structure
biology.protein
Pericyte
Actins/analysis/immunology
Anatomy
Pulmonary alveolus
Myofibroblast
Zdroj: Journal of Histochemistry and Cytochemistry, Vol. 40, No 12 (1992) pp. 1955-1963
ISSN: 0022-1554
Popis: Frozen or paraffin-embedded human and rat lung specimens were stained with antibodies against total actin, alpha-smooth muscle (SM) actin, vimentin, desmin, or gelsolin. Alveolar interstitial myofibroblasts [i.e., contractile interstitial cells (CIC)] were labeled by total actin antibody but not by alpha-SM actin antibody. They stained for vimentin and gelsolin and, in rat lungs, most of them for desmin. Pericytes located around venules at the junction of three alveolar septa were always positive for alpha-SM actin and never for desmin. Tissue samples were also immunostained by an alpha-SM actin antibody and studied by electron microscopy. With this technique we confirmed that cells, identified as pericytes on the basis of their location, were intensely labeled by alpha-SM actin antibodies, whereas alveolar myofibroblasts were not. We conclude that in the lung interstitium pericytes and alveolar myofibroblasts have distinct cytoskeletal features, alpha-SM actin antibody staining being a simple method to distinguish between them. Furthermore, it appears that alveolar myofibroblasts have a peculiar pattern of cytoskeletal protein composition which, in the rat, is similar to that previously described for stromal cells in uterine submucosa, liver sinusoids (Ito cells), or the core of intestinal villi.
Databáze: OpenAIRE
Externí odkaz: