Placental maternally expressed gene 3 differentially methylated region methylation profile is associated with maternal glucose concentration and newborn birthweight

Autor: Min Lv, Ying Jiang, Ting Yan, Yuan Chen, Baihui Zhao, Cheng Chen, Qiong Luo, Mengmeng Yang, Juefei Lu, Fangfang Xi
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Adult
Blood Glucose
Male
Endocrinology
Diabetes and Metabolism
Real-Time Polymerase Chain Reaction
Gestational diabetes mellitus
Diseases of the endocrine glands. Clinical endocrinology
Epigenesis
Genetic
Andrology
03 medical and health sciences
0302 clinical medicine
Pregnancy
Diabetes mellitus
Placenta
Internal Medicine
medicine
Birth Weight
Humans
Pregnancy-Associated Plasma Protein-A
Epigenetics
business.industry
Infant
Newborn
Gestational age
General Medicine
Methylation
Articles
DNA Methylation
medicine.disease
RC648-665
Gestational diabetes
Diabetes
Gestational
030104 developmental biology
medicine.anatomical_structure
Clinical Science and Care
030220 oncology & carcinogenesis
Case-Control Studies
DNA methylation
Linear Models
Original Article
Female
Maternally expressed gene 3 differentially methylated region
Genomic imprinting
business
Zdroj: Journal of Diabetes Investigation, Vol 12, Iss 6, Pp 1074-1082 (2021)
Journal of Diabetes Investigation
ISSN: 2040-1116
2040-1124
Popis: Aims/Introduction Emerging evidence shows that epigenetic modifications occurring during fetal development in response to intrauterine exposures could be one of the mechanisms involved in the early determinants of adult metabolic disorders. This study aimed to investigate whether the placental maternally expressed gene 3 (MEG3) deoxyribonucleic acid (DNA) methylation profile is associated with maternal gestational diabetes mellitus status and newborn birthweight. Materials and Methods Samples for measurement were collected from 23 women with gestational diabetes mellitus and 23 healthy controls. MEG3 gene expression and DNA methylation levels were assessed using quantitative real‐time polymerase chain reaction and MethylTargetTM, respectively. Pearson correlation analyses were used to examine associations between placental DNA methylation levels and clinical variables of interest. The associated results were adjusted by multivariate linear regression for maternal age, body mass index, height, gestational age and newborn sex as confounders. Results We found that the DNA methylation levels in the MEG3 differentially methylated region were significantly different between the gestational diabetes mellitus and control groups on the maternal side of the placenta (40.64 ± 2.15 vs 38.33 ± 2.92; P = 0.004). Furthermore, the mean MEG3 DNA methylation levels were correlated positively with maternal fasting glucose concentrations (R = 0.603, P
The placental deoxyribonucleic acid methylation status in the maternally expressed gene 3 differentially methylated region was correlated with maternal fasting glucose concentrations and the newborn birthweight. These epigenetic adaptations might contribute to late‐onset obesity, underlining the adverse intrauterine environment.
Databáze: OpenAIRE
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