Hesperidin prevents hyperglycemia in diabetic rats by activating the insulin receptor pathway
| Autor: | Li Liu, Yu-Bo Han, Yanbo Sui, Peng Peng, Juan Jin, Dapeng Zhao, Guoliang Zou |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cancer Research medicine.medical_specialty Glucose uptake Impaired glucose tolerance 03 medical and health sciences chemistry.chemical_compound Hesperidin 0302 clinical medicine Insulin resistance Immunology and Microbiology (miscellaneous) Alloxan Diabetes mellitus Internal medicine medicine biology business.industry Glucokinase Articles General Medicine medicine.disease Insulin receptor 030104 developmental biology Endocrinology chemistry 030220 oncology & carcinogenesis biology.protein business |
| Zdroj: | Exp Ther Med |
| ISSN: | 1792-1015 1792-0981 |
| DOI: | 10.3892/etm.2020.9485 |
| Popis: | Diabetes, a disease with high prevalence in China, is a major risk factor of cardiovascular disease. Hesperidin is a flavanone glycoside with anti-hyperglycemic and anti-hyperlipidemic activities. Therefore, the present study aimed to investigate the potential preventive effect of hesperidin against type 2 diabetes mellitus (T2DM) using a rat model of alloxan and high fat diet (HFD)-induced insulin resistance. Male Sprague Dawley rats were orally administered with 100 mg/kg hesperidin or vehicle (sodium carboxy methyl cellulose) for 35 days. Insulin resistance was induced by feeding animals a HFD for 3 weeks (from day 7) and then with an alloxan injection on day 28. Results from the in vivo study demonstrated that hesperidin improved fasting serum glucose (from 19.8 to 10.6 mmol/l) without changing the fasting insulin level, suggesting that hesperidin prevented the development of insulin resistance and diabetes by improving insulin sensitivity. In the oral glucose tolerance test, the development of impaired glucose tolerance was also prevented by hesperidin treatment. Hesperidin was found to regulate glycolysis and gluconeogenesis by enhancing the activity of glucokinase, inducing the phosphorylation of insulin receptor (IR) and phosphoinositide-dependent kinase 1 (PDK1), while decreasing the activity of glucose-6-phosphatase and phosphoenolpyruvate carboxykinase in the liver. In a cell-based assay, hesperidin increased glucose uptake in primary rat adipocytes. Collectively, the present study identified the potent preventive effect of hesperidin against HFD-induced insulin resistance by activating the IR/PDK1 pathway. The current results may provide a potential strategy lacking sides effects to improve metabolic health and reduce risks. |
| Databáze: | OpenAIRE |
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