Fluorescence Intensity- and Lifetime-Based Glucose Sensing Using Glucose/Galactose-Binding Protein
| Autor: | Jonathan Coulter, Faaizah Khan, Zheng-Liang Zhi, John C. Pickup, David J. S. Birch |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Blood Glucose
Fluorophore Monosaccharide Transport Proteins Endocrinology Diabetes and Metabolism Biomedical Engineering Glucose sensing Bioengineering Biosensing Techniques Galactose-binding protein Fluorescence chemistry.chemical_compound In vivo Diabetes Mellitus Fluorescence Resonance Energy Transfer Internal Medicine Humans Blood Glucose Self-Monitoring Calcium-Binding Proteins Fluorescence intensity Glucose Förster resonance energy transfer Biochemistry chemistry Periplasmic Binding Proteins Biophysics Fluorescent glucose biosensor Research Article |
| Zdroj: | Journal of Diabetes Science and Technology. 7:62-71 |
| ISSN: | 1932-2968 |
| DOI: | 10.1177/193229681300700108 |
| Popis: | We review progress in our laboratories toward developing in vivo glucose sensors for diabetes that are based on fluorescence labeling of glucose/galactose-binding protein. Measurement strategies have included both monitoring glucose-induced changes in fluorescence resonance energy transfer and labeling with the environmentally sensitive fluorophore, badan. Measuring fluorescence lifetime rather than intensity has particular potential advantages for in vivo sensing. A prototype fiber-optic-based glucose sensor using this technology is being tested.Fluorescence technique is one of the major solutions for achieving the continuous and noninvasive glucose sensor for diabetes. In this article, a highly sensitive nanostructured sensor is developed to detect extremely small amounts of aqueous glucose by applying fluorescence energy transfer (FRET). A one-pot method is applied to produce the dextran-fluorescein isothiocyanate (FITC)-conjugating mesoporous silica nanoparticles (MSNs), which afterward interact with the tetramethylrhodamine isothiocyanate (TRITC)-labeled concanavalin A (Con A) to form the FRET nanoparticles (FITC-dextran-Con A-TRITC@MSNs). The nanostructured glucose sensor is then formed via the self-assembly of the FRET nanoparticles on a transparent, flexible, and biocompatible substrate, e.g., poly(dimethylsiloxane). Our results indicate the diameter of the MSNs is 60 ± 5 nm. The difference in the images before and after adding 20 μl of glucose (0.10 mmol/liter) on the FRET sensor can be detected in less than 2 min by the laser confocal laser scanning microscope. The correlation between the ratio of fluorescence intensity, I(donor)/I(acceptor), of the FRET sensor and the concentration of aqueous glucose in the range of 0.04–4 mmol/liter has been investigated; a linear relationship is found. Furthermore, the durability of the nanostructured FRET sensor is evaluated for 5 days. In addition, the recorded images can be converted to digital images by obtaining the pixels from the resulting matrix using Matlab image processing functions. We have also studied the in vitro cytotoxicity of the device. The nanostructured FRET sensor may provide an alternative method to help patients manage the disease continuously. |
| Databáze: | OpenAIRE |
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