Simultaneous isolation and label‐free identification of bacteria using contactless dielectrophoresis and Raman spectroscopy
| Autor: | Cynthia Hanson, Jacob T Barney, Elizabeth Vargis, Morgan M. Bishop |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Electrophoresis
Materials science Clinical Biochemistry 02 engineering and technology Spectrum Analysis Raman 01 natural sciences Biochemistry Mycobacterium Analytical Chemistry Agar plate symbols.namesake Humans Electrodes Label free Chromatography Fouling biology 010401 analytical chemistry Electric Conductivity Signal Processing Computer-Assisted Equipment Design Dielectrophoresis 021001 nanoscience & nanotechnology Isolation (microbiology) biology.organism_classification 0104 chemical sciences Electrode symbols Polystyrenes 0210 nano-technology Raman spectroscopy Bacteria |
| Zdroj: | ELECTROPHORESIS. 40:1446-1456 |
| ISSN: | 1522-2683 0173-0835 |
| DOI: | 10.1002/elps.201800389 |
| Popis: | The traditional bacterial identification method of growing colonies on agar plates can take several days to weeks to complete depending on the growth rate of the bacteria. Successfully decreasing this analysis time requires cell isolation followed by identification. One way to decrease analysis time is by combining dielectrophoresis (DEP), a common technique used for cell sorting and isolation, and Raman spectroscopy for cell identification. DEP-Raman devices have been used for bacterial analysis, however, these devices have a number of drawbacks including sample heating, cell-to-electrode proximity that limits throughput and separation efficiency, electrode fouling, or inability to address sample debris. Presented here is a contactless DEP-Raman device to simultaneously isolate and identify particles from a mixed sample while avoiding common drawbacks associated with other DEP designs. Using the device, a mixed sample of bacteria and 3 μm polystyrene spheres were isolated from each other and a Raman spectrum of the trapped bacteria was acquired, indicating the potential for cDEP-Raman devices to decrease the analysis time of bacteria. |
| Databáze: | OpenAIRE |
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