Effect of Natural Vs. Synthetic α-tocopherol on Neurogenesis-Related Genes in Cerebella of Juvenile α-tocopherol Transfer Protein-Null Mice
| Autor: | John W. Erdman, Matthew J. Kuchan, Katherine M. Ranard |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Maternal
Perinatal and Pediatric Nutrition medicine.medical_specialty Cerebellum Nutrition and Dietetics Chemistry Vitamin E medicine.medical_treatment Neurogenesis Medicine (miscellaneous) Myelin medicine.anatomical_structure Endocrinology Internal medicine medicine Tocopherol Transfer technique Neural development Gene Food Science |
| Zdroj: | Curr Dev Nutr |
| Popis: | OBJECTIVES: Vitamin E (α-tocopherol, α-T) restriction during brain development alters the expression of neurogenesis-related genes in cerebella of juvenile α-tocopherol transfer protein-null (Ttpa(−/−)) mice. Synthetic α-T (SYN), compared to natural α-T (NAT), downregulates cerebellar myelin genes in adolescent Ttpa(−/−) mice. We studied how early-life exposure to SYN or NAT affects the expression of neurogenesis-related genes in juvenile Ttpa(−/−) mice. METHODS: Male and female Ttpa(+/+) and Ttpa(−/−) mice were nursed by Ttpa(+/−)dams fed AIN-93G-based diets containing either SYN (∼816 mg α-T/kg diet) or NAT (∼600 mg α-T/kg diet). Homogenized brain tissues from 21 day old weanlings (n = 9/group) were used to measure total α-T concentrations via HPLC-PDA. The expression of genes critical for brain development (Rora, Shh), myelination (Plp1, Cntnap1, Mbp, Mobp, Nr1h3), and synaptic function (Cplx1, Necab1, Prkcg) were measured in the cerebellum via real-time qPCR. RESULTS: α-T concentrations were significantly lower in brains of Ttpa(−/−) mice (17.7 nmol/g) compared to Ttpa(+/+) mice (37.5 nmol/g) (P |
| Databáze: | OpenAIRE |
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