Profiling of extracellular vesicle-bound miRNA to identify candidate biomarkers of chronic alcohol drinking in non-human primates
| Autor: | Sloan A. Lewis, Brianna Doratt, Suhas Sureshchandra, Tianyu Pan, Steven W. Gonzales, Weining Shen, Kathleen A. Grant, Ilhem Messaoudi |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Alcohol Drinking nonhuman primates Clinical Sciences Medicine (miscellaneous) Down-Regulation Toxicology Cardiovascular Oral and gastrointestinal Article Dose-Response Relationship Alcohol Use and Health Substance Misuse Extracellular Vesicles Genetics 2.1 Biological and endogenous factors Psychology Animals Humans Aetiology miRNA Cancer Dose-Response Relationship Drug Ethanol alcohol Prevention Neurosciences Substance Abuse Macaca mulatta Stroke Psychiatry and Mental health Alcoholism MicroRNAs Good Health and Well Being Female Drug Biomarkers Biotechnology |
| Zdroj: | Alcohol Clin Exp Res Alcoholism, clinical and experimental research, vol 46, iss 2 |
| Popis: | BACKGROUND: Long-term alcohol drinking is associated with numerous health complications including susceptibility to infection, cancer, and organ damage. However, due to the complex nature of human drinking behavior, it has been challenging to identify reliable biomarkers of alcohol use that could be used to determine drinking behavior prior to signs of overt organ damage. Recently, extracellular vesicle-bound microRNA (EV-miRNA) have been discovered to be consistent biomarkers of conditions including cancer and liver disease. METHODS: In this study, we profiled the plasma EV-miRNA content by miRNA-Seq from 80 non-human primates after 12 months of voluntary ethanol drinking. RESULTS: We identified a list of up- and downregulated EV-miRNA candidate biomarkers of both heavy drinking as well as those positively correlated with ethanol dose. We further overexpressed these candidate miRNA in control primary peripheral immune cells to assess potential functional mechanisms of these EV-miRNA. We report that overexpression of miR-155, miR-154, miR-34c, miR-450a, and miR-204 led to increased inflammatory TNFα or IL-6 production in PBMC after stimulation. CONCLUSION: This exploratory study identified several EV-miRNA that could serve as biomarkers of long-term alcohol drinking as well as provided a mechanism for alcohol-induced peripheral inflammation. |
| Databáze: | OpenAIRE |
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