Evidence for a mitochondrial ATP-regulated potassium channel in human dermal fibroblasts

Autor: Agnieszka Walewska, Michal Laskowski, Wieslawa Jarmuszkiewicz, Adam Szewczyk, Rafal P. Kampa, Bogusz Kulawiak, Milena Krajewska, Piotr Bednarczyk, Anna Kicinska
Rok vydání: 2017
Předmět:
Zdroj: Biochimica et biophysica acta. Bioenergetics. 1859(5)
ISSN: 0005-2728
Popis: Mitochondrial ATP-regulated potassium channels are present in the inner membrane of the mitochondria of various cells. In the present study, we show for the first time mitochondrial ATP-regulated potassium channels in human dermal fibroblast cells. Using the patch-clamp technique on the inner mitochondrial membrane of fibroblasts, we detected a potassium channel with a mean conductance equal to 100 pS in symmetric 150 mM KCl. The activity of this channel was inhibited by a complex of ATP/Mg 2+ and activated by potassium channel openers such as diazoxide or BMS 191095. Channel activity was inhibited by antidiabetic sulfonylurea glibenclamide and 5-hydroxydecanoic acid. The influence of substances modulating ATP-regulated potassium channel activity on oxygen consumption and membrane potential of isolated fibroblast mitochondria was also studied. Additionally, the potassium channel opener diazoxide lowered the amount of superoxide formed in isolated fibroblast mitochondria. Using reverse transcriptase-PCR, we found an mRNA transcript for the KCNJ1(ROMK) channel. The presence of ROMK protein was observed in the inner mitochondrial membrane fraction. Moreover, colocalization of the ROMK protein and a mitochondrial marker in the mitochondria of fibroblast cells was shown by immunofluorescence. In summary, the ATP-regulated mitochondrial potassium channel in a dermal fibroblast cell line have been identified.
Databáze: OpenAIRE
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