Serological diagnosis and prognosis of severe acute pancreatitis by analysis of serum glycoprotein 2
| Autor: | Hans-Ulrich Schulz, Christian Hentschel, Pamela Holzlöhner, Alexander Goihl, Dirk Roggenbuck, Katja Hanack, Peter Schierack, Dirk Reinhold, Miklos Veiczi |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male medicine.medical_specialty Clinical Biochemistry GPI-Linked Proteins Likelihood ratios in diagnostic testing Gastroenterology Procalcitonin Biliary disease Cohort Studies 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Zymogen granule membrane Humans Protein Isoforms Serologic Tests Aged business.industry Biochemistry (medical) General Medicine Middle Aged medicine.disease Prognosis Pancreatitis Gastrointestinal disease 030220 oncology & carcinogenesis Case-Control Studies Acute Disease Acute pancreatitis Institut für Chemie 030211 gastroenterology & hepatology Female Differential diagnosis business Blood Chemical Analysis |
| Zdroj: | Clinical chemistry and laboratory medicine. 55(6) |
| ISSN: | 1437-4331 |
| Popis: | Background:Glycoprotein 2 (GP2), the pancreatic major zymogen granule membrane glycoprotein, was reported to be elevated in acute pancreatitis in animal models.Methods:Enzyme-linked immunosorbent assays (ELISAs) were developed to evaluate human glycoprotein 2 isoform alpha (GP2a) and total GP2 (GP2t) as specific markers for acute pancreatitis in sera of 153 patients with acute pancreatitis, 26 with chronic pancreatitis, 125 with pancreatic neoplasms, 324 with non-pancreatic neoplasms, 109 patients with liver/biliary disease, 67 with gastrointestinal disease, and 101 healthy subjects. GP2a and GP2t levels were correlated with procalcitonin and C-reactive protein in 152 and 146 follow-up samples of acute pancreatitis patients, respectively.Results:The GP2a ELISA revealed a significantly higher assay accuracy in contrast to the GP2t assay (sensitivity ≤3 disease days: 91.7%, specificity: 96.7%, positive likelihood ratio [LR+]: 24.6, LR–: 0.09). GP2a and GP2t levels as well as prevalences were significantly elevated in early acute pancreatitis (≤3 disease days) compared to all control cohorts (pρ)=0.21, 0.26; p=0.0110, 0.0012; respectively] and C-reactive protein (ρ=0.37, 0.40; pConclusions:Serum GP2a is a specific marker of acute pancreatitis and analysis of GP2a can aid in the differential diagnosis of acute upper abdominal pain and prognosis of severe acute pancreatitis. |
| Databáze: | OpenAIRE |
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