The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions
| Autor: | Egor Dzyubenko, Christine Gottschling, Andreas Faissner, Maren Geissler |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
General Immunology and Microbiology General Chemical Engineering General Neuroscience Synaptogenesis Hippocampus Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Chemically defined medium 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure nervous system Cell culture medicine Neuroglia Neuron Neuroscience 030217 neurology & neurosurgery Function (biology) Astrocyte |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| DOI: | 10.3791/54757-v |
| Popis: | Proper neuronal development and function is the prerequisite of the developing and the adult brain. However, the mechanisms underlying the highly controlled formation and maintenance of complex neuronal networks are not completely understood thus far. The open questions concerning neurons in health and disease are diverse and reaching from understanding the basic development to investigating human related pathologies, e.g., Alzheimer's disease and Schizophrenia. The most detailed analysis of neurons can be performed in vitro. However, neurons are demanding cells and need the additional support of astrocytes for their long-term survival. This cellular heterogeneity is in conflict with the aim to dissect the analysis of neurons and astrocytes. We present here a cell-culture assay that allows for the long-term cocultivation of pure primary neurons and astrocytes, which share the same chemically defined medium, while being physically separated. In this setup, the cultures survive for up to four weeks and the assay is suitable for a diversity of investigations concerning neuron-glia interaction. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|