Immunohistopathologic evaluation of choroidal neovascular membranes following Verteporfin-photodynamic therapy
| Autor: | Faik Gelisken, B. A. Lafaut, Salvatore Grisanti, Karl Ulrich Bartz-Schmidt, Werner Inhoffen, Jolanta Oficjalska-Mlynczak, Olcay Tatar, Peter Szurman, Serap Canbek, Sabin Aisenbrey |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Pathology medicine.medical_specialty Porphyrins medicine.medical_treatment CD34 Vascular Cell Adhesion Molecule-1 Antigens CD34 Receptors Cell Surface Photodynamic therapy Capillary Permeability Immunoenzyme Techniques Neovascularization Macular Degeneration Antigens CD Humans Medicine Fluorescein Angiography Aged Retrospective Studies Aged 80 and over Photosensitizing Agents medicine.diagnostic_test Choroid business.industry Endoglin Verteporfin Middle Aged Macular degeneration Fluorescein angiography medicine.disease Fibrosis Choroidal Neovascularization eye diseases Ophthalmology Ki-67 Antigen medicine.anatomical_structure Photochemotherapy Female Endothelium Vascular medicine.symptom business Perfusion medicine.drug |
| Zdroj: | American Journal of Ophthalmology. 137:914-923 |
| ISSN: | 0002-9394 |
| DOI: | 10.1016/j.ajo.2003.12.049 |
| Popis: | Purpose To evaluate the vascularization and proliferative activity in choroidal neovascular membranes due to age-related macular degeneration after verteporfin photodynamic therapy and submacular removal. Design Interventional case series. Methods In a retrospective review of seven patients who underwent removal of subfoveal classic choroidal neovascular membranes after treatment with photodynamic therapy 3 to 146 days earlier, membranes were stained for CD 34, CD 105, and Ki-67 and correlated with clinical pictures and fluorescein angiography. Results Fluorescein angiography performed on the day of surgery disclosed nonperfusion of the treated area 3 days after photodynamic therapy, but perfusion and leakage were seen at greater post–photodynamic therapy intervals. Membranes excised 3 days after photodynamic therapy showed CD34 and CD105 positive, mostly occluded vessels. The endothelial cells appeared damaged. Ki-67 activity was low. In membranes excised 34 to 146 days after photodynamic therapy, all vessels appeared patent and were lined by healthy endothelial cells with strong expression of CD34 and CD105. Ki-67 expression was elevated after 34 days but decreased thereafter. Conclusion Photodynamic therapy did not cause a general or complete occlusion of vessels within the choroidal neovascular membranes, as suggested by fluorescein angiography 3 days postintervention, but the endothelial cells appeared to be severely damaged. Proliferative activity within these specimens was reduced. At longer intervals after photodynamic therapy, the fibrovascular tissue seemed to recover; perfusion, hyperfluorescence, and leakage of the choroidal neovascular membranes could be detected by fluorescein angiography. The clinical appearance showed a correlation with the immunohistologic characteristics of an increased proliferative activity and patent vascularization. |
| Databáze: | OpenAIRE |
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