Porin alteration and active efflux: two in vivo drug resistance strategies used by Enterobacter aerogenes
| Autor: | Charléric Bornet, Jacqueline Chevalier, Annie Eyraud, C. Bollet, A. Davin-Regli, Monique Malléa, Jean-Marie Pagès |
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| Rok vydání: | 1998 |
| Předmět: |
Cell Membrane Permeability
biology Membrane permeability Enterobacter Enterobacteriaceae Infections Porins Drug Resistance Microbial Microbial Sensitivity Tests Drug resistance Quinolones beta-Lactams Enterobacter aerogenes biology.organism_classification Microbiology Enterobacteriaceae Drug Resistance Multiple beta-Lactamases Anti-Bacterial Agents Multiple drug resistance Biochemistry Porin Humans Efflux Antibacterial agent |
| Zdroj: | Microbiology. 144:3003-3009 |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/00221287-144-11-3003 |
| Popis: | SUMMARY: Entembacter aemgenes is among the five most frequently isolated nosocomial pathogens in France, and this bacterium also shows increasing multidrug resistance. In this study, various E. aerogenes strains isolated from hospital units were characterized for their outer-membrane proteins, antibiotic susceptibilities (inhibition diameters and MICs) and resistance mechanisms associated with modification of envelope permeability (porin alteration and active efflux). Diminished outer-membrane permeability due to porin alterations was found in conjunction with the expression of an enzymic barrier in resistant isolates. Interestingly, changes in the functional expression of porins appeared to play a special role in susceptibility to cefepime. An active efflux to quinolones was also identified. Simultaneous changes in envelope permeability, i.e. a porin deficiency (in) and an efflux mechanism (out), were clearly evident in two clinical strains. |
| Databáze: | OpenAIRE |
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