Glycinergic input of small-field amacrine cells in the retinas of wildtype and glycine receptor deficient mice
| Autor: | Gregory A. O'Sullivan, L. Heinze, H.-X. Chen, Heinz Wässle, Heinrich Betz, J. Weiss |
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| Rok vydání: | 2008 |
| Předmět: |
Patch-Clamp Techniques
Glycine Biology Inhibitory postsynaptic potential Retina Membrane Potentials GABA Antagonists Mice Cellular and Molecular Neuroscience chemistry.chemical_compound Receptors Glycine medicine Animals Drug Interactions Patch clamp Molecular Biology Glycine receptor Mice Knockout Dose-Response Relationship Drug Glycine Agents Strychnine Cell Biology GABA receptor antagonist Electric Stimulation Cell biology Mice Inbred C57BL Amacrine Cells medicine.anatomical_structure Inhibitory Postsynaptic Potentials chemistry Biochemistry Linear Models |
| Zdroj: | Molecular and Cellular Neuroscience. 37:40-55 |
| ISSN: | 1044-7431 |
| DOI: | 10.1016/j.mcn.2007.08.012 |
| Popis: | Amacrine cells are known to express strychnine-sensitive glycine receptors (GlyRs), however, it is not known which of the four GlyRalpha subunits (alpha1-4) are expressed in this diverse group of cells. Herein, we studied the presence of glycine activated currents and spontaneous inhibitory postsynaptic currents (sIPSCs) of amacrine cells in the mouse retina. By recording glycinergic currents in retinal slices of wildtype mice and of mice deficient in GlyRalpha subunits (Glra1spd-ot, Glra2-/-, Glra3-/-), we could classify AII and narrow-field amacrine cells (NF, Types 5, 6, 7) on the basis of their alpha-subunit composition. Glycinergic sIPSCs of AII cells displayed medium fast kinetics (mean decay time constant tau=11+/-2 ms), which were completely absent in the Glra3-/- mouse, indicating that synaptic GlyRs of AII cells mainly contain the alpha3 subunit. Glycinergic sIPSCs of NF cells had slow kinetics (tau=27+/-6.8 ms) that were significantly prolonged in Glra2-/- mice (tau=69+/-16 ms). These data show that morphologically distinct amacrine cells express different sets of GlyRs. |
| Databáze: | OpenAIRE |
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