Application of newly developed SARS-CoV2 serology test along with real-time PCR for early detection in health care workers and on-time plasma donation
Autor: | Hossein Samadi Kafil, Mahsa Hajivalili, Abolfazl Miahipour, Amin Kamrani, Javad Ahmadian Heris, Mohammad Sadegh Soltani-Zangbar, Forough Parhizkar, Ramin Pourakbari, Leili Aghebati-Maleki, Oldouz Shareghi-Oskoue, Leila Roshangar, Homayoon Siahmansouri, Sara Farhang, Farhad Jadidi-Niaragh, Roza Motavalli, Maryam Hosseini, Mehdi Yousefi, Mostafa Haji-Fatahaliha, Ata Mahmoodpoor |
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Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Saliva IgM IgG ELISA Enzyme Linked Immune-Sorbent Assay Spike protein medicine.disease_cause Virus Article Serology 03 medical and health sciences RBD receptor binding domain 0302 clinical medicine PCR polymerase chain reaction Antigen S spike protein Genetics Medicine Coronavirus Nucleocapsid protein biology business.industry Antibody titer Virology N nucleocapsid protein 030104 developmental biology Real-time polymerase chain reaction 030220 oncology & carcinogenesis SARS-CoV2 biology.protein SARS-CoV-2 Severe Acute Respiratory Syndrome caused by Coronavirus-2 ELISA Antibody business Receptor binding domain |
Zdroj: | Gene Reports |
ISSN: | 2452-0144 |
Popis: | Background As the daily number of coronavirus infection disease 19 (COVID19) patients increases, the necessity of early diagnosis becomes more obvious. In this respect, we aimed to develop a serological test for specifically detecting anti-SARS-CoV2 antibodies. Methods We collected serum and saliva samples from 609 individuals who work at TBZMED affiliated hospitals in Tabriz, Iran, from April to June of 2020. Real-time PCR technique was used to detect SARS-CoV-2 genome using specific primers. An enzyme linked immunosorbent assay (ELISA) test was designed based on virus nucleocapsid (N), spike (S) and its receptor binding domain (RBD) protein, and the collected sera were subjected to IgM and/or IgG analysis. Result Real-time PCR results showed that 66 people were infected with the SARS-CoV-2. Our designed ELISA kit showed 93.75% and 98% of sensitivity and specificity, respectively. In this study, 5.74% of participants had specific IgG against RBD, whereas the percentage for IgM positive individuals was 5.58%. Approximately the same results were observed for S protein. The number of positive participants for NP increased further, and the results of this antigen showed 7.38% for IgG and 7.06% for IgM. Conclusion The ELISA test beside real-time PCR could provide a reliable serologic profile for the status of the disease progress and early detection of individuals. More importantly, it possesses the potential to identify the best candidates for plasma donation according to the antibody titers. |
Databáze: | OpenAIRE |
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