Laccase immobilized peroxidase mimicking magnetic metal organic frameworks for industrial dye degradation
| Autor: | Prasad Gajanan Belokar, Mayur R. Ladole, Pravin B. Pokale, Sujata S. Patil, Aniruddha B. Pandit |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Environmental Engineering Materials science Bioengineering 010501 environmental sciences 01 natural sciences 010608 biotechnology Industrial dye degradation Thermal stability Coloring Agents Waste Management and Disposal Metal-Organic Frameworks 0105 earth and related environmental sciences Peroxidase Laccase Renewable Energy Sustainability and the Environment Magnetic Phenomena General Medicine Enzymes Immobilized Chemical engineering Biocatalysis Degradation (geology) Magnetic nanoparticles Metal-organic framework Particle size |
| Zdroj: | Bioresource technology. 317 |
| ISSN: | 1873-2976 |
| Popis: | In the present work, laccase was successfully immobilized in peroxidase mimicking magnetic metal organic frameworks (MMOFs) within 30 min using a facile approach. The integration of magnetic nanoparticles during synthesis significantly eases the separation of prepared biocatalyst using an external magnet. The immobilization of laccase was confirmed using different characterization techniques. The laccase@MMOFs found spherical in nature with an average particle size below 100 nm. The synthesized laccase embedded framework exhibits supermagnetic property with the saturation magnetization (Ms) of 34.12 emu/gm. The prepared bio-metallic frameworks maintain high surface area and thermal stability. The laccase@MMOFs was successfully exploited for the degradation of industrial dyes in batch and continuous mode with an average degradation efficiency of 95%. The prepared laccase structure had an excellent recyclability retaining upto 89% residual activity upto 10th cycle and can be stored at room temperature upto 30 days without any significant loss of activity. |
| Databáze: | OpenAIRE |
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