Role of lymphokines in regulation of macrophage differentiation
| Autor: | Kaoru Miura, Tatsuichiro Hashimoto, Kiyoko S. Akagawa, Shinji Haga, Kikuo Onozaki, Tohru Tokunaga |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
medicine.medical_specialty
Guinea Pigs Immunology Leukemia Inhibitory Factor Mice Colony-Stimulating Factors Internal medicine medicine Animals Macrophage Receptor Macrophage Migration-Inhibitory Factors Immunosorbent Techniques Glycoproteins Lymphokines biology Interleukin-6 Macrophages Lymphokine Myeloid leukemia Cell Differentiation Macrophage Activation Molecular biology Growth Inhibitors In vitro Endocrinology Macrophage-Activating Factors Concanavalin A biology.protein Macrophage migration inhibitory factor Antibody |
| Zdroj: | Cellular Immunology. 76:129-136 |
| ISSN: | 0008-8749 |
| Popis: | The regulatory mechanism of guinea pig lymphokines was investigated in regard to differentiation of myeloid cells to macrophages. The Ml-cell line, established from a myeloid leukemia of an SL-strain mouse, was induced to differentiate in vitro into mature macrophages possessing Fc receptors and the ability to phagocytize latex particles by treatment with crude lymphokines. Both concanavalin A- and antigen-induced lymphokines showed the differentiation-inducing factor (D factor) activity. However, macrophage migration inhibitory factor/ macrophage activation factor (MIF/MAF) purified by an immunoadsorbent column with anti-MIF antibody had no such an activity. The D-factor activity was detected in the lymphokine preparation that was not retained on the immunoadsorbent column. In contrast, colony-stimulating factor (CSF) was adsorbed to the immunoadsorbent column, and could be recovered in the purified MIF/MAF preparation. These findings suggest that the molecular entity of D factor is distinct from MIF/ MAF and CSF. A culture supernatant of guinea pig peritoneal macrophages activated with MIF/ MAF (CSF) exhibited strong D-factor activity. However, the supernatant possessed rather reduced CSF activity as compared to that of the original MIF/MAF (CSF) preparation. Thus, MIF/MAF may play an important role in macrophage differentiation by regulating the production of D factor or CSF from macrophages. |
| Databáze: | OpenAIRE |
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