8-Methoxypsoralen photoadduct formation in complementary oligonucleotides containing a cross-linkable site
| Autor: | Francis P. Gasparro, J. Park, L. A. Amici |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Guanine
UVA Radiation Light Stereochemistry Ultraviolet Rays Immunology Oligonucleotides Dermatology Tritium High-performance liquid chromatography chemistry.chemical_compound Cytosine DNA Adducts Immunology and Allergy Radiology Nuclear Medicine and imaging Chromatography High Pressure Liquid Base Composition Photosensitizing Agents Chemistry Oligonucleotide Adenine General Medicine Reversed-phase chromatography Cross-Linking Reagents Helix Methoxsalen DNA Thymine |
| Zdroj: | Photodermatology, photoimmunologyphotomedicine. 11(3) |
| ISSN: | 0905-4383 |
| Popis: | The complete profile of 8-methoxypsoralen photoadduct formation in complementary oligonucleotides (5'-GAGTATGAG and 5'-CATAC) has been determined. Equimolar solutions of the oligonucleotides were irradiated at 4 degrees C in order to stabilize the mini-double helix. Photomodified oligonucleotides were separated by reversed phase chromatography on a Vydac C4 column. Photoadduct formation favored the 5'TAT site in the 9mer over the 5'ATA site in the 5mer by a factor of two. Split-dose studies showed that the monoadducts formed on GAGTATGAG were preferentially converted to cross-links by an additional UVA exposure. |
| Databáze: | OpenAIRE |
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